The role of indigenous microflora in the development of murine intestinal fucosyl- and sialyltransferases
The role of indigenous microflora in the development of murine intestinal fucosyl- and sialyltransferases
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DOI：
10.1096/fj.02-0031fje
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发表时间：
2002-11-01
期刊：
影响因子：
4.8
通讯作者：
Walker, WA
中科院分区：
文献类型：
--
作者：
Nanthakumar, NN;Dai, DW;Walker, WA
大多数肠道细菌使用肠刷缘糖缀合物作为它们的靶宿主细胞受体。据推测，常驻微生物调节负责合成刷边糖缀合物的特定糖基转移酶。为了验证这一假设，我们测量了成熟常规（CONV）、无菌（GF）和脱无菌（XGF）小鼠肠道不同区域的糖基转移酶活性，并将其与肠道发育的一般酶标志物（如双糖酶）进行了比较。乳母CONV小鼠从十二指肠到结肠均检测到高水平的α 2,3/6-唾液转移酶（ST）活性和低水平的α 1,2- focusyltransferase （FT）活性，但在产后第3周时，这些活性的相对水平发生逆转，并在第4周时迅速达到成虫水平。这些与年龄相关的酶变化在GF小鼠中显著减弱，在3周后保持不成熟模式。GF小鼠肠道菌群的引入迅速启动了糖基转移酶活性的成熟，但对双糖酶的发育程序没有显著影响。因此，在小鼠中，肠道糖基转移酶活性受组织和发育控制，微生物群在其特定的个体发生中起主要作用，而不是在整体发育中起主要作用。这些发现可能有助于解释共生细菌和肠道病原体的区域特异性，也可能将微生物群落的年龄相关变化与肠道病原体的易感性联系起来。
Most enteric bacteria use intestinal brushborder glycoconjugates as their target host cell receptors. It has been postulated that resident microbes regulate specific glycosyltransferases that are responsible for synthesizing brushborder glycoconjugates. To investigate this hypothesis, we measured glycosyltransferase enzyme activities in intestine from different regions of maturing conventional (CONV), germ-free (GF), and ex-germ-free (XGF) mice and compared them to general enzyme markers of gut development, for example, disaccharidases. High alpha2,3/6-Sialyltransferase (ST) activity and low alpha1,2-fucosyltransferase (FT) activities were detected from duodenum to colon in suckling CONV mice, but the relative levels of these activities reversed during the third postnatal wk, rapidly reaching adult levels by the fourth wk. These age-related enzyme changes were significantly attenuated in GF mice, maintaining an immature pattern well past 3 wk. Introduction of gut microflora in GF mice rapidly initiated maturation of glycosyltransferase activity but had no significant affect on developmental programming of dissacharidases. Therefore, in mice, intestinal glycosyltransferase activities are under tissue and developmental control and microflora play a major role in their specific ontogeny but not in overall development. These findings may help explain the regional specificity of commensal bacteria and of enteric pathogens and may also relate age-related changes in microflora to susceptibility to enteropathogens.