ISOLATION OF GLYCOPROTEINS FROM PIG LYMPHOCYTE PLASMA-MEMBRANE USING LENS-CULINARIS PHYTOHEMAGGLUTININ
ISOLATION OF GLYCOPROTEINS FROM PIG LYMPHOCYTE PLASMA-MEMBRANE USING LENS-CULINARIS PHYTOHEMAGGLUTININ
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DOI：
10.1016/0006-291x(72)90581-5
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发表时间：
1972-01-01
影响因子：
3.1
通讯作者：
CRUMPTON, MJ
中科院分区：
文献类型：
--
作者：
HAYMAN, MJ;CRUMPTON, MJ
将溶解于1%脱氧胆酸钠溶液中的淋巴细胞质膜糖蛋白选择性地通过LcH *吸附纯化，共价附着于Sepharose 4B，随后用甲基-a- d -甘露吡喃苷洗脱。糖蛋白的产率是使用刀豆蛋白A-Sepharose的两倍以上。纯化的糖蛋白抑制LcH和PHA对淋巴细胞的转化。该方法可能普遍适用于膜糖蛋白的纯化。
Lymphocyte plasma membrane glycoprotiens solubilized in 1% sodium deoxycholate were selectively purified by adsorption to LcH∗, covalently attached to Sepharose 4B, and by subsequent elution with methyl-a-D-mannopyranoside. The yield of glycoprotein was more than twice that obtained using Concanavalin A-Sepharose. The purified glycoproteins inhibited the transformation of lymphocytes by LcH and PHA. The method is probably of general applicability to the purification of membrane glycoproteins.