Dramatic declines in seropositivity as determined with crude extracts of Plasmodium falciparum schizonts between 2000 and 2010 in Dielmo and Ndiop, Senegal
Dramatic declines in seropositivity as determined with crude extracts of Plasmodium falciparum schizonts between 2000 and 2010 in Dielmo and Ndiop, Senegal
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DOI：
10.1186/1475-2875-13-83
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发表时间：
2014-03-06
期刊：
影响因子：
3
通讯作者：
Balde, Aissatou Toure
中科院分区：
文献类型：
--
作者：
Diop, Fode;Richard, Vincent;Balde, Aissatou Toure
背景：塞内加尔自2010年起实施疟疾预消除计划，疟疾负担大幅减轻。应使用有效的工具来监测流行病学的这些变化，以估计传播模式的变化。在塞内加尔疟疾流行程度不同的两个村庄 Dielmo 和 Ndiop，人们对感染情况进行了长达 20 年的纵向跟踪，在此期间采取了多项控制干预措施，导致传播大幅减少。本研究旨在比较 2000 年至 2010 年间这两个村庄居民的疟疾抗体反应，使用当地恶性疟原虫株 (Pf Sch07/03) 的裂殖体粗提物。 方法：从两个村庄的居民采集血清（2000 年从 Dielmo 采集 141 份，从 Ndiop 采集 79 份；2000 年从 Dielmo 采集 143 份，从 Ndiop 采集 79 份血清） 2010）用于评估针对 Pf Sch07/03 裂殖体粗提取物的抗体的流行率。定义了三个年龄组：[5-9]岁、[10-14]岁和[15-19]岁。进行了统计比较。比较不同年龄组、村庄和时期之间的血清阳性率和抗体反应强度。结果：2000 年至 2010 年间，两个村庄的 P.fSch07/03 总体血清阳性率均下降：Dielmo 从 94.4% 降至 44.4%，Ndiop 从 74.4% 降至 34.6%。 Dielmo 和 Ndiop 之间的差异在 2000 年非常显着 (p < 0.001)，但在 2010 年则不显着 (p > 0.20)。年轻居民（超过 40%）的血清阳性率下降幅度大于年长居民（低于 19%）。对年轻群体的纵向监测显示，2000 年至 2010 年间，Dielmo 的血清阳性率从 98.7 下降到 79.3，但 Ndiop 的血清阳性率没有下降，从 67.6 下降到 66.7。两个村庄 2000 年血清阳性个体的抗体反应程度均显着高于 2010 年。结论：恶性疟原虫粗提物是评估不同时期、低流行区和高流行区疟疾流行率的适当工具。使用当地菌株的粗提物来评估传播情况可以有效评估控制计划对疟疾传播的影响。
Background: Programmes of pre-elimination of malaria have been implemented in Senegal since 2010, and the burden of malaria has decreased substantially. These changes in the epidemiology should be monitored with effective tools that allow changes in patterns of transmission to be estimated. In Dielmo and Ndiop, two villages of Senegal with different malaria endemicity, infections have been followed longitudinally for 20 years, during which time there have been several control interventions leading to substantial decreases of transmission. This study aimed to compare malaria antibody responses of the inhabitants of these two villages, between 2000 and 2010, using schizont crude extracts of a local strain of P. falciparum (Pf Sch07/03).Methods: Sera collected from inhabitants of the two villages (141 from Dielmo and 79 from Ndiop in 2000; 143 from Dielmo and 79 from Ndiop in 2010) were used to assess the prevalence of antibodies against crude schizont extracts of Pf Sch07/03. Three ages groups were defined: [5-9] yrs, [10-14] yrs and [15-19] yrs. Statistical comparisons were performed. Seroprevalence and the magnitude of antibody responses were compared between age groups, villages and periods.Results: Overall seroprevalence to P.fSch07/03 decreased between 2000 and 2010 in both villages: from 94.4% to 44.4% in Dielmo and from 74.4% to 34.6% in Ndiop. The difference between Dielmo and Ndiop was highly significant in 2000 (p < 0.001) but not in 2010 (p > 0.20). The decrease in seroprevalence was larger in younger (more than 40%) than older (less than 19%) inhabitants. Longitudinal monitoring of the younger group showed that seroprevalence decreased between 2000 and 2010 in Dielmo from 98.7 to 79.3, but not in Ndiop from 67.6 to 66.7. The magnitude of antibody responses in seropositive individuals was significantly higher in 2000 than 2010 for both villages.Conclusions: Crude extracts of P. falciparum are appropriate tools for evaluating malaria prevalence at different periods, and in both low and high endemic area. Using crude extracts from local strains to assess transmission may allow efficient evaluation of the consequences of control programs on malaria transmission.