Isolation and characterization of the novel Popeye gene family expressed in skeletal muscle and heart
Isolation and characterization of the novel Popeye gene family expressed in skeletal muscle and heart
复制标题
DOI：
10.1006/dbio.2000.9751
复制
发表时间：
2000-07-15
影响因子：
2.7
通讯作者：
Brand, T
中科院分区：
文献类型：
--
作者：
Andrée, B;Hillemann, T;Brand, T
我们在脊椎动物中发现了一个新的基因家族，该基因家族优先在发育中和成年的横纹肌中表达。在人类和小鼠中检测到了大力水手 (POP) 家族的三个基因，在鸡中检测到了两个基因。染色体作图表明，Pop1 和 Pop3 基因聚集在小鼠 10 号染色体上，而 Pop2 则聚集在小鼠 16 号染色体上。我们发现证据表明鸡中的 POP1 和 POP3 也可能是连锁的，并且从该位点产生多个转录亚型。 POP 基因编码具有三个潜在跨膜结构域的蛋白质，这些跨膜结构域在所有家族成员中都是保守的。各个 POP 基因在发育和出生后表现出特定的表达模式。鸡POP3和小鼠Pop1首先优先在心房中表达，随后也在心室的心外膜下致密层中表达。鸡POP1和小鼠Pop2在除流出道外的整个心脏中表达。所有三种 Pop 基因均在成年小鼠的心脏和骨骼肌中表达，在肺中表达较低。 Pop1 和 Pop2 的表达在怀孕小鼠的子宫中上调。与小鼠基因一样，人类 POP 基因主要在骨骼肌和心肌中表达。 POP 基因在进化过程中的高度保守性及其在心脏和骨骼肌中的优先表达表明这些新蛋白可能在脊椎动物的这些组织中具有重要功能。 (C) 2000 年学术出版社。
We identified a novel gene family in vertebrates which is preferentially expressed in developing and adult striated muscle. Three genes of the Popeye (POP) family were detected in human and mouse and two in chicken. Chromosomal mapping indicates that Pop1 and Pop3 genes are clustered on mouse chromosome 10, whereas Pop2 maps to mouse chromosome 16. We found evidence that POP1 and POP3 in chicken may also be linked and multiple transcript isoforms are generated from this locus. The POP genes encode proteins with three potential transmembrane domains that are conserved in all family members. Individual POP genes exhibit specific expression patterns during development and postnatally. Chicken POP3 and mouse Pop1 are first preferentially expressed in atrium and later also in the subepicardial compact layer of the ventricles. Chicken POP1 and mouse Pop2 are expressed in the entire heart except the outflow tract. All three Pop genes are expressed in heart and skeletal muscle of the adult mouse and lower in lung. Pop1 and Pop2 expression is upregulated in uterus of pregnant mice. Like the mouse genes, human POP genes are predominantly expressed in skeletal and cardiac muscle. The strong conservation of POP genes during evolution and their preferential expression in heart and skeletal muscle suggest that these novel proteins may have an important function in these tissues in vertebrates. (C) 2000 Academic Press.