In vitro selection of metal ion-selective DNAzymes.
In vitro selection of metal ion-selective DNAzymes.
复制标题
DOI：
10.1007/978-1-61779-545-9_18
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Ihms, Hannah E;Lu, Yi
DNAzymes 的发现可以在金属离子存在的情况下催化多种反应，无论是在基础层面还是在实践层面都具有重要意义。它增进了我们对金属-核酸相互作用的理解，并允许设计高灵敏度和选择性的金属离子传感器。这一成功的关键因素是一种称为体外选择的技术，它可以快速选择金属特异性 RNA 切割 DNAzyme。体外选择是一个迭代过程，其中包含随机区域的 DNA 池与目标金属离子一起孵育。那些催化优选反应的DNA序列（“获胜者”）被扩增并进行到下一步，在更严格的条件下进行选择。通过这种方式，选择库变得富含具有所需活性和选择性的 DNAzyme。所描述的方法可用于分离对许多不同类型的金属离子或同一金属离子的不同氧化态具有选择性的DNAzyme。
The discovery of DNAzymes that can catalyze a wide range of reactions in the presence of metal ions is important on both fundamental and practical levels; it advances our understanding of metal-nucleic acid interactions and allows for the design of highly sensitive and selective metal ion sensors. A crucial factor in this success is a technique known as in vitro selection, which can rapidly select metal-specific RNA-cleaving DNAzymes. In vitro selection is an iterative process where a DNA pool containing a random region is incubated with the target metal ion. Those DNA sequences that catalyze the preferred reaction (the "winners") are amplified and carried on to the next step, where the selection is carried out under more stringent conditions. In this way, the selection pool becomes enriched with DNAzymes that exhibit desirable activity and selectivity. The method described can be applied to isolate DNAzymes selective to many different types of metal ions or different oxidation states of the same metal ion.