Microarray analysis of BeWo and JEG3 trophoblast cell lines: Identification of differentially expressed transcripts
Microarray analysis of BeWo and JEG3 trophoblast cell lines: Identification of differentially expressed transcripts
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DOI：
10.1016/j.placenta.2006.05.001
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发表时间：
2007-05-01
期刊：
影响因子：
3.8
通讯作者：
Golos, T. G.
中科院分区：
文献类型：
--
作者：
Burleigh, D. W.;Kendziorski, C. M.;Golos, T. G.
滋养细胞系是胎盘功能研究中替代原代滋养细胞的重要研究工具。由于转化滋养层细胞的细胞起源可能是多种多样的，因此在全球范围内了解细胞的独特和共享表型将是有价值的。我们通过微阵列分析比较了两种广泛使用的细胞系BeWo和JEG3，以鉴定差异表达基因。结果表明，大约2700个基因在不同细胞系之间存在差异表达，主要差异体现在对胁迫的反应、细胞粘附、信号转导以及蛋白质和核碱基代谢等生物学过程中。这些数据表明，BeWo和JEG3细胞系，也许还有其他滋养细胞系，彼此之间有足够的差异，因此它们将不同地适合于特定的实验范式。(c) 2006 Elsevier Ltd.版权所有。
Trophoblast cell lines are important research tools used as a surrogate for primary trophoblast cells in the study of placental function. Because the cellular origins of transformed trophoblasts are likely to be diverse, it would be of value to understand the unique and shared phenotypes of the cells on a global scale. We have compared two widely used cell lines, BeWo and JEG3, by microarray analysis in order to identify differentially expressed genes. Results indicated that approximately 2700 genes were differentially expressed between the cell lines, with principal differences observed in the biological processes of response to stress, cell adhesion, signal transduction, and protein and nucleobase metabolisms. These data suggest that BeWo and JEG3 cell lines, and perhaps other trophoblast cell lines, are sufficiently dissimilar from each other such that they will be differentially suited for specific experimental paradigms. (c) 2006 Elsevier Ltd. All rights reserved.