Metabolism, excretion and distribution of the flame retardant tetrabromobisphenol-A in conventional and bile-duct cannulated rats
Metabolism, excretion and distribution of the flame retardant tetrabromobisphenol-A in conventional and bile-duct cannulated rats
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DOI：
10.1080/004982500433309
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发表时间：
2000-09-01
期刊：
影响因子：
1.8
通讯作者：
Örn, U
中科院分区：
文献类型：
--
作者：
Hakk, H;Larsen, G;Örn, U
1.C-14-TBBP-A（2，2-双（4-羟基-3，5-二溴苯基）丙烷）经口给予常规和胆管插管的雄性Sprague-Dawley大鼠（2.0 mg/kg体重）。每天收集尿液、胆汁和粪便，共收集72 h，并取出选定的组织进行分布研究.粪便是常规大鼠中TBBP-A的主要消除途径（91.7%的剂量），尿液是次要消除途径（0.3%）。在胆管插管大鼠中，71.3%的给药放射性通过胆汁排泄，26.7%的给药放射性通过粪便排泄，表明存在肝肠循环。组织中的C-14标记残留物在常规大鼠中为2%，在胆管插管大鼠中<1%。两组大鼠的大肠和小肠含有大部分组织C-14活性。肝脏中TBBP-A含量<0.1%，脂肪中TBBP-A含量低于定量水平.在0-24 h胆汁样品中表征了三种代谢产物。葡糖醛酸和硫酸酯共轭物的特点是通过质谱。超过95%的可提取粪便C-14被鉴定为母体TBBP-A。TBBP-A衍生的C-14的量可忽略不计，与尿液和胆汁中的载体蛋白相关。
1.C-14-TBBP-A (2,2-bis(4-hydroxy-3,5-dibromophenyl)propane) was administered orally to the conventional and bile-duct cannulated male Sprague-Dawley rat (2.0 mg/kg body weight). Urine, bile and faeces were collected daily for 72 h, and selected tissues were removed for distribution studies.2. Faeces was the major route of elimination of TBBP-A in the conventional rat (91.7 % of dose), and urine was a minor elimination route (0.3 %). Enterohepatic circulation was suggested by biliary excretion of 71.3 % and faecal excretion of 26.7 % of the administered radioactivity in the bile-duct cannulated rat.3. C-14-labelled residues in tissues were 2 % in the conventional rat, and < 1 % in the bile-duct cannulated rat. The large and small intestines contained the majority of the tissue C-14 activity for both groups of rat. Levels of TBBP-A in liver were < 0.1 %, and in fat were below the level of quantification.4. Three metabolites were characterized in 0-24 h bile samples. Glucuronic acid and sulphate ester conjugates were characterized by mass spectrometry. More than 95 % of the extractable faecal C-14 was identified as parent TBBP-A.5. Negligible amounts of TBBP-A-derived C-14 were associated with carrier proteins in the urine and bile.