In vivo analysis of interactions between GFP-labeled microfilaments and plastid stromules.
In vivo analysis of interactions between GFP-labeled microfilaments and plastid stromules.
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DOI：
10.1186/1471-2229-4-2
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发表时间：
2004-02-10
影响因子：
5.3
通讯作者：
Hanson MR
中科院分区：
文献类型：
--
作者：
Kwok EY;Hanson MR
质体基质是高等植物中从质体表面延伸出来的充满基质的小管，允许质体之间进行蛋白质分子的交换。这些结构是高度动态的;基质在细胞质中的形状和位置变化非常迅速。以前的研究与微丝抑制剂表明，子实体的形状和运动是依赖于肌动蛋白细胞骨架。为了更多地了解基质和细胞骨架相互作用的性质，我们对荧光标记的微丝和质体进行了成像。我们利用表达与人类肌动蛋白结合蛋白talin融合的绿色荧光蛋白的拟南芥植物观察微丝及其与体内基质的关系。下胚轴表皮的基质和质体与微丝紧密接触。时间推移共聚焦显微镜显示，微丝重排与质体和子实体的形态和位置的变化。我们还观察到在双标记细胞中线粒体和基质之间的密切相互作用。我们的研究结果表明，微丝的重排和质体和基质的形状和位置的变化之间的相关性。基质与微丝相互作用，微丝也可以被线粒体和其他细胞器利用。微丝和质体的相互作用可能是由质体被膜上的肌动蛋白结合蛋白介导的。
Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromules change both their shape and position in the cytoplasm very rapidly. Previous studies with microfilament inhibitors indicated that stromule shape and movement are dependent on the actin cytoskeleton. To learn more about the nature of the interactions of stromules and the cytoskeleton, we imaged fluorescently-labeled microfilaments and plastids. We have used Arabidopsis thaliana plants expressing green fluorescent protein fused to the human actin-binding protein talin to observe microfilaments and their relationship to stromules in vivo. Microfilaments were observed in close contact with stromules and plastid bodies of hypocotyl epidermis. Time-lapse confocal microscopy revealed that microfilament rearrangements were associated with changes in plastid and stromule morphology and position. We also observed close interactions between mitochondria and stromules in double-labeled cells. Our results indicate a correlation between the rearrangement of microfilaments and changes in the shape and position of plastids and stromules. Stromules interact with microfilaments that may also be utilized by mitochondria and other organelles. The interaction of microfilaments and plastids is likely to be mediated by actin-binding proteins on the plastid envelope membrane.