Recent advances in blood-related proteomics
Recent advances in blood-related proteomics
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DOI：
10.1002/pmic.200402053
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发表时间：
2005-08-01
期刊：
影响因子：
3.4
通讯作者：
Tissot, JD
中科院分区：
文献类型：
--
作者：
Thadikkaran, L;Siegenthaler, MA;Tissot, JD
血液分为两个隔室，即血浆和细胞。后者含有红细胞、白细胞和血小板。血液学从一门描述性的医学学科发展成为一门开拓性的学科，其中分子生物学允许开发疾病的预后和诊断指标。最近在MS和蛋白质分离方面的进展使蛋白质的分析也有了类似的进展。蛋白质组学为血浆/血清中蛋白质的研究提供了广阔的前景，事实上已经建立了许多血浆/血清蛋白质组学数据库。这是一种非常复杂的体液，含有脂类、碳水化合物、氨基酸、维生素、核酸、激素和蛋白质。最近已经确定了大约1500种不同的蛋白质，并鉴定了一些潜在的疾病新标记物。在这里，给出了血浆/血清蛋白质组分析在诊断/预后标志物和疾病机制信息方面的巨大前景的例子。在血液中也有大量不同的血细胞类型，它们可能含有类似的信息。到目前为止，红细胞蛋白质组学并没有改善我们对这些细胞的了解，这与在研究血小板和白细胞方面取得的重大进展形成了鲜明对比。未来，蛋白质组学将改变血液学的几个方面。
Blood is divided in two compartments, namely, plasma and cells. The latter contain red blood cells, leukocytes, and platelets. From a descriptive medical discipline, hematology has evolved towards a pioneering discipline where molecular biology has permitted the development of prognostic and diagnostic indicators for disease. The recent advance in MS and protein separation now allows similar progress in the analysis of proteins. Proteomics offers great promise for the study of proteins in plasma/serum, indeed a number of proteomics databases for plasma/serum have been established. This is a very complex body fluid containing lipids, carbohydrates, amino acids, vitamins, nucleic acids, hormones, and proteins. About 1500 different proteins have recently been identified, and a number of potential new markers of diseases have been characterized. Here, examples of the enormous promise of plasma/serum proteomic analysis for diagnostic/prognostic markers and information on disease mechanism are given. Within the blood are also a large number of different blood cell types that potentially hold similar information. Proteomics of red blood cells, until now, has not improved our knowledge of these cells, in contrast to the major progresses achieved while studying platelets and leukocytes. in the future, proteomics will change several aspects of hematology.