Proteomics of Shewanella oneidensis MR-1 biofilm reveals differentially expressed proteins, including AggA and RibB
Proteomics of Shewanella oneidensis MR-1 biofilm reveals differentially expressed proteins, including AggA and RibB
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DOI：
10.1002/pmic.200400989
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发表时间：
2005-04-01
期刊：
影响因子：
3.4
通讯作者：
Van Beeumen, J
中科院分区：
文献类型：
--
作者：
De Vriendt, K;Theunissen, S;Van Beeumen, J
希瓦氏菌MR-1是革兰氏阴性的兼性需氧细菌，能够呼吸多种电子受体。由于其具有还原固体铁的能力，因此在微生物引起的金属表面腐蚀中起着重要作用。由于这需要细胞粘附在金属表面，生物膜的生长是这一过程的基本特征。本研究的目的是通过二维凝胶电泳比较无根和浮游生长的水蛭细胞的整体蛋白表达模式。质谱法作为鉴定差异表达蛋白的工具。采用pH 3-10和pH 4-7的IPG条带进行等电聚焦。对二维图形的分析共指出了59个相关点。在这些蛋白质中，我们强调了一种被注释为凝集蛋白（AggA）的蛋白质的参与。AggA是一种类似tolc的蛋白，可能是ABC转运蛋白的一部分。另一种差异表达蛋白是RibB，一种核黄素生物合成途径的酶。核黄素是黄素单核苷酸（FMN）和黄素腺嘌呤二核苷酸（FAD）的前体分子，可能是生物膜细胞相对于浮游细胞呼吸特性改变所必需的。鉴定出的一些蛋白质表明生物膜处于厌氧状态。这种厌氧的生活方式影响了细胞的能量获取途径，并通过几种蛋白质的存在反映出来，包括血红素利用系统的蛋白质。
Shewanella oneidensis MR-1 is a Gram-negative, facultative aerobic bacterium, able to respire a variety of electron acceptors. Due to its capability to reduce solid ferric iron, S. oneidensis plays an important role in microbially induced corrosion of metal surfaces. Since this requires cellular adhesion to the metal surface, biofilm growth is an essential feature of this process. The goal of this work was to compare the global protein expression patterns of sessile and planktonic grown S. oneidensis cells by two-dimensional (2-D) gel electrophoresis. Mass spectrometry was used as an identification tool of the differentially expressed proteins. An IPG strip of pH 3-10 as well as pH 4-7 was applied for iso-electrofocusing. Analysis of the 2-D patterns pointed out a total of 59 relevant spots. Among these proteins, we highlight the involvement of a protein annotated as an agglutination protein (AggA). AggA is a TolC-like protein which is presumably part of an ABC transporter. Another differentially expressed protein is RibB, an enzyme of the riboflavin biosynthesis pathway. Riboflavin is the precursor molecule of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) and may be necessary for the altered respiratory properties of the biofilm cells versus planktonic cells. Some proteins that were identified indicate an anaerobic state of the biofilm. This anaerobic way of living affects the energy gaining pathways of the cell and is reflected by the presence of several proteins, including those of a heme-utilization system.