Upregulation of endothelial nitric oxide synthase in rat aorta after ingestion of fish oil-rich diet
Upregulation of endothelial nitric oxide synthase in rat aorta after ingestion of fish oil-rich diet
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DOI：
10.1152/ajpheart.01145.2003
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发表时间：
2004-08-01
影响因子：
4.8
通讯作者：
Mitjavila, MT
中科院分区：
文献类型：
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作者：
López, D;Orta, X;Mitjavila, MT
先前的一项研究表明，从喂食富含鱼油的食物的大鼠身上分离出的主动脉段表明，乙酰胆碱诱导的一氧化氮（一氧化氮）增加。没有)介导的放松。然而，富含鱼油的饮食是否会影响血管活动本身和穴位，这一点仍有待阐明。鱼油作用的NO-cGMP途径。为此目的。两组Sprague-Dawley大鼠分别饲喂含5%脂质玉米油（CO）或鲱鱼油（MO）的半纯化饲料8周。研究内皮NO合成酶（eNOS） mRNA和蛋白水平及NOS活性。血管的生物利用度。用电子自旋共振光谱法直接测定NO。cGMP、l -精氨酸和l -瓜氨酸的水平也在匀浆中进行了评估。研究了主动脉段超氧阴离子（O-2）的产生及其抗氧化活性。大鼠主动脉组织eNOS mRNA含量升高。这导致eNOS蛋白水平（相对于喂食富含co饮食的大鼠70%）和NOS活性（102%）增加；．NO产量增加了90%，cGMP水平增加了100%，l -精氨酸减少了30%。主动脉O-2（-）无变化。膳食MO诱导eNOS-cGMP通路的上调可能有助于维持血管稳态，并解释其在预防动脉疾病中的有益作用。
A previous study with aortic segments isolated from rats fed a fish oil-rich diet indicated an increase in acetylcholine-induced nitric oxide (.NO)-mediated relaxation. However, it remained to be elucidated whether a fish oil-rich diet affects the vascular activity per se and the point of the .NO-cGMP pathway at which fish oil acts. For this purpose. two groups of Sprague-Dawley rats were fed a semipurified diet containing 5% lipids, either corn oil (CO) or menhaden oil (MO), for 8 wk. We Studied the mRNA and protein levels of endothelial NO synthase (eNOS) and NOS activity. The bioavailability of vascular .NO was assessed directly by electron spin resonance spectroscopy. The levels of cGMP, L-arginine, and L-Citrulline were also evaluated in homogenates. Superoxide anion (O-2(-).) production and related antioxidant activities were also studied in aortic segments. The aortic content of eNOS mRNA was increased in rats fed the MO-rich diet. This resulted in increases in both eNOS protein levels (70% relative to the rats fed the CO-rich diet) and NOS activity (102%); .NO production increased by 90%, cGMP levels increased by 100%, and L-arginine decreased by 30%. No change in aortic O-2(-). production was caused by dietary MO. The Upregulation of the eNOS-cGMP pathway induced by dietary MO may contribute to the maintenance of vascular homeostasis and explain its beneficial effect in the prevention of arterial diseases.