Avian leukosis virus-induced osteopetrosis is associated with the persistent synthesis of viral DNA.
Avian leukosis virus-induced osteopetrosis is associated with the persistent synthesis of viral DNA.
复制标题
禽白血病病毒引起的石骨症与病毒 DNA 的持续合成有关。
DOI：
10.1016/0042-6822(85)90189-8
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Miles,BD
中科院分区：
文献类型：
--
作者：
Robinson,HL;Miles,BD
从19例禽白血病病毒引起的骨硬化症的DNA进行了分析的病毒序列。其中包括快速、中间和缓慢发作的骨硬化症。骨硬化症骨的DNA中没有证据表明骨硬化症是由前病毒插入宿主原癌基因或病毒转导宿主原癌基因引起的。相反，来自骨硬化症的骨的DNA显示出骨硬化症与病毒DNA的持续合成相关的证据。19个DNA中的每一个都含有未整合的以及整合的病毒DNA。快速发作的骨硬化症比中、晚发性骨硬化症含有约3倍多的病毒和前病毒DNA。未整合的病毒DNA不能检测到DNA提取的法氏囊和骨髓的骨硬化症的鸡或DNA提取的正常骨的禽白血病病毒感染的鸡。因此，持续合成未整合的病毒DNA中观察到骨硬化，但不是正常组织的禽白血病病毒感染的鸡。
DNAs from 19 cases of avian leukosis virus-induced osteopetrosis have been analyzed for viral sequences. Among these were instances of rapid, intermediate, and slow onset osteopetrosis. The DNAs from osteopetrotic bone contained no evidence for osteopetrosis being caused by proviral insertions into or viral transductions of a host protooncogene. Instead, DNAs from osteopetrotic bone displayed evidence for osteopetrosis being associated with the persistent synthesis of viral DNA. Each of the 19 DNAS contained unintegrated as well as integrated viral DNA. Rapid onset osteopetrosis contained about 3× more viral and proviral DNA than intermediate or late onset osteopetrosis. Unintegrated viral DNA could not be detected in DNAs extracted from the bursa and bone marrow of osteopetrotic chickens or in DNA extracted from the normal bones of an avian leukosis virus-infected chicken. Thus, the persistent synthesis of unintegrated viral DNA was observed in osteopetrotic but not normal tissues of avian leukosis virus-infected chickens.