Pinpoint Diagnostic Kit for Heat Stroke by Monitoring Lysosomal pH.
Pinpoint Diagnostic Kit for Heat Stroke by Monitoring Lysosomal pH.
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DOI：
10.1021/acs.analchem.7b03612
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发表时间：
2017-10
影响因子：
7.4
通讯作者：
Ying Wen;Weijie Zhang;Tao Liu;Fangjun Huo;C. Yin
中科院分区：
文献类型：
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作者：
Ying Wen;Weijie Zhang;Tao Liu;Fangjun Huo;C. Yin
中暑是最严重的死亡原因之一。为了防止这种情况的发生，研究热的细胞毒性机制是至关重要的。初步结果表明，中暑与溶酶体酸度的变化有一定的相关性。为了进一步阐明它们之间的关系，在这里，我们报告了一种高选择性和高灵敏度的荧光探针(NT1)，用于开启对pH值的传感。合成NT2作为对照化合物。与NT2相比，NT1具有准确的溶酶体靶向能力。此外，合适的pKa值(5.67)允许NT1对溶酶体pH值的变化做出反应。更重要的是，NT1可用于研究溶酶体pH变化与中暑的相关性。结果表明，中暑时，溶酶体的pH值随温度升高而升高。因此，NT1是研究中暑复杂生物学机制的一个很好的候选基因。
Heat stroke is one of the most serious causes of mortality. To prevent the situation, it is fundamental to research the mechanism of heat cytotoxicity. The preliminary results revealed that heat stroke and the change of lysosome acidity had some certain correlation. To further clarify their relationship, herein, we report a highly selective and sensitive fluorescence probe (NT1) for turn-on sensing of the pH value. NT2 was synthesized as control compound. Compared to NT2, NT1 showed accurate lysosome target ability. In addition, the suitable pKa value (5.67) allows NT1 to response to the changes of lysosomal pH values. Most importantly, NT1 could be used to study the correlation between the change of lysosomal pH and heat stroke. It was shown that the lysosomal pH value increasing with temperature during heat stroke. Thus, NT1 was an excellent candidate for research of the complex biological mechanism of heat stroke.