The action of certain antibiotics on mitochondrial, erythrocyte and artificial phospholipid membranes. The role of induced proton permeability.
The action of certain antibiotics on mitochondrial, erythrocyte and artificial phospholipid membranes. The role of induced proton permeability.
复制标题
某些抗生素对线粒体、红细胞和人工磷脂膜的作用。
作者：
Peter J. F. Henderson;J. McGivan;J. Chappell
1.本文研究了抗生素恩镰孢菌素A、缬氨霉素、肌动蛋白同系物短杆菌肽、尼日利亚菌素和戴那霉素对卵磷脂-双鲸蜡基磷酸氢盐的线粒体、红细胞和近晶相的作用。2.这些抗生素诱导渗透性的碱金属阳离子的所有三个膜系统。3.各膜系统的离子特异性相同。4. Enniatin A，缬氨霉素和肌动蛋白不诱导渗透质子，而尼日利亚菌素和dianemycin使所有三个膜系统自由渗透质子。5.尼日利亚菌素诱导的渗透性和短杆菌肽诱导的渗透性之间存在一些差异。6.所有这些抗生素对线粒体呼吸的作用可以通过线粒体膜的被动离子渗透性的变化来解释，这些变化类似于在红细胞和磷脂膜中诱导的变化，如果假设膜电位存在于呼吸线粒体中的话。
1. The action of the antibiotics enniatin A, valinomycin, the actin homologues, gramicidin, nigericin and dianemycin on mitochondria, erythrocytes and smectic mesophases of lecithin-dicetyl hydrogen phosphate was studied. 2. These antibiotics induced permeability to alkali-metal cations on all three membrane systems. 3. The ion specificity on each membrane system was the same. 4. Enniatin A, valinomycin and the actins did not induce permeability to protons, whereas nigericin and dianemycin rendered all three membrane systems freely permeable to protons. 5. Several differences were noted between permeability induced by nigericin and that induced by gramicidin. 6. The action of all these antibiotics on mitochondrial respiration could be accounted for by changes in passive ion permeability of the mitochondrial membrane similar to those induced in erythrocytes and phospholipid membranes, if it is assumed that a membrane potential is present in respiring mitochondria.