Connexin43 and Angiotensin II Alterations in Hearts of Rats Having Undergone an Acute Exposure to Alcohol
Connexin43 and Angiotensin II Alterations in Hearts of Rats Having Undergone an Acute Exposure to Alcohol
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DOI：
10.1097/paf.0b013e31827bf67f
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发表时间：
2013-03-01
影响因子：
1
通讯作者：
Liu, Shui-Ping
中科院分区：
文献类型：
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作者：
Huang, Quan-Yong;Li, Xue-Fang;Liu, Shui-Ping
前言：酒精引起的心脏损伤与酶和蛋白质的改变有关。本研究旨在探讨急性酒精中毒对大鼠心脏连接蛋白43（Cx43）和血管紧张素Ⅱ（Ang Ⅱ）的影响。乙醇组腹腔注射乙醇3.8g/kg，对照组腹腔注射等量生理盐水。翻正反射消失后，对所有动物进行胸骨正中切开术。免疫组化法检测Cx43和Ang Ⅱ蛋白表达。结果：急性乙醇处理后，Cx43表达明显减少，积分光密度值较对照组明显降低（P < 0.05）。与对照组相比，急性乙醇处理后Ang II的表达显著增加，积分光密度支持（P < 0.05）。结论：总之，急性乙醇处理后，发现心脏蛋白Cx43和Ang II的表达显著改变，提示这2种蛋白可能是急性乙醇期间心脏易受氧化损伤的重要潜在机制。本研究表明，急性乙醇中毒引起不同的心脏蛋白质的变化，这将与氧化应激。
Introduction: Alcohol-induced heart damage is associated with enzyme and protein alterations. The purpose of this study was to investigate alcohol-induced alterations in cardiac connexin 43 (Cx43) and angiotensin II (Ang II) after acute alcohol administration.Method: Male Wistar rats were randomly divided into 2 groups: a control group and an ethanol group. The ethanol group intraperitoneally received 3.8 g/kg ethanol; the controls were given the same amount of saline via the same route. After the righting reflex disappeared, midsternotomy was performed in all animals. Immunohistochemical analysis was performed to evaluate protein expression of Cx43 and Ang II. Sections were analyzed by digital image analysis.Result: The expression of Cx43 was significantly reduced after acute ethanol treatment, with the integrated optical density lower when compared with control (P < 0.05). The expression of Ang II was significantly increased after acute ethanol treatment, supported by integrated optical density when compared with control (P < 0.05).Conclusions: In summary, cardiac protein expression of Cx43 and Ang II were found to be significantly altered after acute ethanol treatment, suggesting that these 2 proteins may be important underlying mechanisms of vulnerability to oxidative injury in the heart during acute ethanol. The present study indicated that acute ethanol toxicity caused different alterations in heart proteins that would be related to oxidative stress.