Long-term effects of culture of preimplantation mouse embryos on behavior
Long-term effects of culture of preimplantation mouse embryos on behavior
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DOI：
10.1073/pnas.0306846101
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发表时间：
2004-02-10
影响因子：
11.1
通讯作者：
Schultz, RM
中科院分区：
文献类型：
--
作者：
Ecker, DJ;Stein, P;Schultz, RM
在辅助生殖技术（ART）中用于治疗人类不育症的许多程序需要培养植入前胚胎。此外，在子宫移植前将胚胎培养更长时间以确定用于移植的“最佳”胚胎并尽量减少多胎妊娠的趋势越来越明显。然而，胚胎培养会干扰胚胎的代谢和基因表达，培养的长期后果尚不清楚。我们使用129 S6/SvEvTac/C57 BL/6 J F-1小鼠模型探索了胚胎培养的行为后果，发现培养胚胎衍生的成年小鼠在高架零迷宫和Morris水迷宫任务中表现出特定的行为改变。
Many procedures used in assisted reproductive technologies (ART) to treat human infertility entail culture of preimplantation embryos. Moreover, there is an increasing trend to culture embryos for longer periods of time before uterine transfer to identify the "best" embryos for transfer and to minimize multiple pregnancies. Embryo culture, however, can perturb embryo metabolism and gene expression, and the long-term consequences of culture are unknown. We have explored the behavioral consequences of embryo culture by using a 129S6/SvEvTac/C57BL/6J F-1 mouse model and find that adults derived from cultured embryos exhibit specific behavioral alterations in the elevated zero maze and Morris water maze tasks.