Use of Speckle Tracking Echocardiography to Detect Induced Regional Strain Changes in the Murine Myocardium by Acoustic Radiation Force.
Use of Speckle Tracking Echocardiography to Detect Induced Regional Strain Changes in the Murine Myocardium by Acoustic Radiation Force.
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DOI：
10.4250/jcvi.2020.0148
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发表时间：
2021-04
影响因子：
--
通讯作者：
Kohut A
中科院分区：
文献类型：
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作者：
Chaudhury A;Wanek A;Ponnalagu D;Singh H;Kohut A
缺血性冠状动脉疾病（ischemic coronary artery disease，CAD）是心力衰竭（heart failure，HF）的先兆，在动物模型中模拟CAD引起的心肌应变异常是一个困难的问题。对这些应变变化的模拟可能有助于更好地了解HF的早期形成阶段。这在研究射血分数保留性心力衰竭（HFpEF）的发病机制方面尤为重要。在这里，我们讨论了高强度聚焦超声（HIFU）在小鼠模型中的传递，以改变左心室（LV）区域纵向应变（RLS），并使用斑点跟踪超声心动图来检测这些变化。通过将来自函数发生器的正弦波形放大到压电换能器中来产生HIFU脉冲（压力振幅1.7MPa）。然后在三个时间段（早期、中期和晚期）将这些脉冲在体外指向C57BI6小鼠的LV前表面。斑点追踪超声心动图，然后被用来量化的RLS在六个节段的LV内的变化。在所有三个时间段内，我们观察到声学增强的LV RLS增加。这种增强在早期脑白质的前顶区附近和晚期脑白质的后基底区附近最为突出。我们的研究结果表明，高强度聚焦超声的应用，非侵入性地诱导小鼠模型内的RLS的变化。我们的研究结果也反映了斑点追踪超声心动图分析和量化这些变化的能力。这些发现代表了在小动物模型中超声诱导的LV RLS增强的首次证明。
It is difficult to simulate the abnormal myocardial strain patterns caused by ischemic coronary artery disease (CAD) which are a precursor to heart failure (HF) within an animal model. Simulation of these strain changes could contribute to better understanding of the early formative stages of HF. This is especially important in investigating the poorly understood pathogenesis of heart failure with preserved ejection fraction (HFpEF). Here, we discuss delivery of high intensity focused ultrasound (HIFU) in a murine model to alter left ventricular (LV) regional longitudinal strain (RLS), and use of speckle tracking echocardiography to detect these changes. HIFU pulses (pressure amplitude 1.7 MPa) were generated by amplifying a sinusoidal waveform from a function generator into a piezoelectric transducer. These pulses were then directed extracorporeally towards the anterior LV surface of C57BI6 mice during three time periods (early, mid, and late diastole). Speckle tracking echocardiography was then used to quantify changes in RLS within six segments of the LV. We observed an increase in LV RLS with acoustic augmentation during all three time periods. This augmentation was most prominent near the anterior apical region in early diastole and near the posterior basilar region during late diastole. Our findings demonstrate the application of HIFU to non-invasively induce changes in RLS within a murine model. Our results also reflect the capability of speckle tracking echocardiography to analyze and quantify these changes. These findings represent the first demonstration of ultrasound-induced augmentation in LV RLS within a small animal model.