Association of Kidney Function with Changes in the Endothelial Surface Layer
Association of Kidney Function with Changes in the Endothelial Surface Layer
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DOI：
10.2215/cjn.08160813
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发表时间：
2014-04-07
影响因子：
9.8
通讯作者：
Rabelink, Ton J.
中科院分区：
文献类型：
--
作者：
Dane, Martijn J. C.;Khairoun, Meriem;Rabelink, Ton J.
背景与目的终末期肾病（ESRD）伴有内皮功能障碍。由于内皮糖萼（内皮表面层）控制流动的血液和血管壁之间的相互作用，扰动可能会影响疾病的进展。本研究采用了一种新的非侵入性侧流暗场成像方法，该方法测量了微循环中红细胞对内皮表面层的可及性设计、设置、参与者和测量在对照参与者（n=10）、患有ESRD的患者（n=23）、成功的活体供肾移植后肾功能正常的参与者（n=12）和肾移植后发生间质纤维化/肾小管萎缩的患者（n=10）。结果与健康对照组（1.82 ± 0.16 μ m）相比，ESRD患者的血流灌注边界区（+0.23; 95%可信区间，0.46 ~ 0.16 μ m）明显增大，而对照组（1.82 ± 0.16 μ m）明显增大（+0.23; 95%可信区间，0.46 ~ 0.16 μ m），ESRD患者的血流灌注边界区（+0.23; 95%可信区间，0.46 ~ 0.16 μ m）明显增大（P < 0.05）。
Background and objectivesESRD is accompanied by endothelial dysfunction. Because the endothelial glycocalyx (endothelial surface layer) governs interactions between flowing blood and the vessel wall, perturbation could influence disease progression. This study used a novel noninvasive sidestream-darkfield imaging method, which measures the accessibility of red blood cells to the endothelial surface layer in the microcirculation (perfused boundary region), to investigate whether renal function is associated with endothelial surface layer dimensions.Design, setting, participants, & measurementsPerfused boundary region was measured in control participants (n=10), patients with ESRD (n=23), participants with normal kidney function after successful living donor kidney transplantation (n=12), and patients who developed interstitial fibrosis/tubular atrophy after kidney transplantation (n=10). In addition, the endothelial activation marker angiopoietin-2 and shed endothelial surface layer components syndecan-1 and soluble thrombomodulin were measured using ELISA.ResultsCompared with healthy controls (1.820.16 mu m), ESRD patients had a larger perfused boundary region (+0.23; 95% confidence interval, 0.46 to