The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins
The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins
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DOI：
10.1016/s0092-8674(00)81290-8
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发表时间：
1996-02-09
期刊：
影响因子：
64.5
通讯作者：
Jones, JDG
中科院分区：
文献类型：
--
作者：
Dixon, MS;Jones, DA;Jones, JDG
在植物中，对病原体的抗性通常由显性抗性基因决定，其产物被提议识别病原体编码的无毒基因（Avr）产物。通过定位克隆的方法分离到番茄抗病基因座Cf-2，发现该基因座含有两个几乎相同的基因，每个基因都赋予番茄叶霉病（C. fulvum）表达相应的Avr 2基因。这两个Cf-2基因编码的蛋白质产物彼此仅相差3个氨基酸，并含有38个富含亮氨酸的重复序列（LRR）基序。在LRR中，20个显示出极其保守的交替重复。Cf-2的C-末端携带与由未连接的Cf-9基因编码的蛋白质具有明显同源性的区域。我们认为，这个保守的区域与其他参与激活植物防御机制的蛋白质相互作用。
In plants, resistance to pathogens is frequently determined by dominant resistance genes, whose products are proposed to recognize pathogen-encoded avirulence gene (Avr) products. The tomato resistance locus Cf-2 was isolated by positional cloning and found to contain two almost identical genes, each conferring resistance to isolates of tomato leaf mould (C. fulvum) expressing the corresponding Avr2 gene. The two Cf-2 genes encode protein products that differ from each other by only three amino acids and contain 38 leucine-rich repeat (LRR) motifs. Of the LRRs, 20 show extremely conserved alternating repeats. The C-terminus of Cf-2 carries regions of pronounced homology to the protein encoded by the unlinked Cf-9 gene. We suggest that this conserved region interacts with other proteins involved in activating plant defense mechanisms.