Identification of differentially expressed genes in the kidneys of growth hormone transgenic mice
Identification of differentially expressed genes in the kidneys of growth hormone transgenic mice
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DOI：
10.1016/j.ghir.2010.06.001
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发表时间：
2010-10-01
影响因子：
1.4
通讯作者：
Kopchick, J. J.
中科院分区：
文献类型：
--
作者：
Coschigano, K. T.;Wetzel, A. N.;Kopchick, J. J.
目的：牛生长激素(BGH)转基因小鼠发生严重的肾脏损害。这种损害可能至少部分是由于基因表达的变化。设计：采用基因消减文库、Northern印迹分析、基因芯片分析和实时逆转录聚合酶链式反应(RT/PCR)等方法，鉴定和验证BGH小鼠肾脏与非转基因(NT)小鼠肾脏RNA表达差异的特异性基因。结果：BGH小鼠和非转基因(NT)小鼠肾脏差异表达基因绝大多数为免疫球蛋白。几个糖蛋白基因和炎症相关基因在BGH肾脏中的RNA表达也增加。相比之下，只有几个基因在BGH肾脏中被鉴定为显著下调。最显著的是肾脏雄激素调节蛋白基因的表达下降。结论：在BGH肾脏中发现了一些差异表达的基因。免疫球蛋白和炎症相关基因包括两组，表明免疫系统在BGH肾脏损害中发挥了作用。(C)2010年生长激素研究会。爱思唯尔有限公司出版。保留所有权利。
Objective: Bovine growth hormone (bGH) transgenic mice develop severe kidney damage. This damage may be due, at least in part, to changes in gene expression. Identification of genes with altered expression in the bGH kidney may identify mechanisms leading to damage in this system that may also be relevant to other models of kidney damage.Design: cDNA subtraction libraries, northern blot analyses, microarray analyses and real-time reverse transcription polymerase chain reaction (RT/PCR) assays were used to identify and verify specific genes exhibiting differential RNA expression between kidneys of bGH mice and their non-transgenic (NT) littermates.Results: Immunoglobulins were the vast majority of genes identified by the cDNA subtractions and the microarray analyses as being up-regulated in bGH. Several glycoprotein genes and inflammation-related genes also showed increased RNA expression in the bGH kidney. In contrast, only a few genes were identified as being significantly down-regulated in the bGH kidney. The most notable decrease in RNA expression was for the gene encoding kidney androgen-regulated protein.Conclusions: A number of genes were identified as being differentially expressed in the bGH kidney. Inclusion of two groups, immunoglobulins and inflammation-related genes, suggests a role of the immune system in bGH kidney damage. (C) 2010 Growth Hormone Research Society. Published by Elsevier Ltd. All rights reserved.