Normalization of tyrosine hydroxylase activity in vivo in the striatum of transgenic mice carrying human tyrosine hydroxylase gene: a microdialysis study
Normalization of tyrosine hydroxylase activity in vivo in the striatum of transgenic mice carrying human tyrosine hydroxylase gene: a microdialysis study
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携带人酪氨酸羟化酶基因的转基因小鼠纹状体内酪氨酸羟化酶活性的正常化：微透析研究
DOI：
10.1016/0304-3940(93)90608-n
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发表时间：
1993
影响因子：
2.5
通讯作者：
T. Nagatsu
中科院分区：
文献类型：
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作者：
D. Nakahara;H. Hashiguti;N. Kaneda;T. Sasaoka;T. Nagatsu
使用微透析方法，我们观察到与非转基因小鼠相比，在NSD-1015灌注（10− 4 M）后，携带人酪氨酸羟化酶（TH）基因的转基因小鼠纹状体中有类似的稳态3，4-二羟基苯丙氨酸积累。在灌注NSD-1015之前，3，4-二羟基苯乙酸的基础细胞外水平在转基因和非转基因纹状体中也是相当的。结果提示，尽管转基因小鼠纹状体TH蛋白表达增加，但其体内活性仍通过某种调节机制保持在正常水平。
Using a microdialysis method, we observed a similar steady-statel-3,4-dihydroxyphenylalanine accumulation in the striatum of transgenic mice carrying the human tyrosine hydroxyIase (TH) gene after NSD-1015 perfusion (10−4M) as compared to nontransgenic mice. Basal extracellular levels of 3,4-dihydroxyphenylacetic acid before the perfusion of NSD-1015 were also comparable in both transgenic and non-transgenic striata. The results suggest that the in vivo activity of TH in the striatum of transgenic mice was retained to the normal level by some regulatory mechanism(s) in spite of the increased expression of the enzyme protein.
K.Kiuchi：“携带人酪氨酸羟化酶基因的转基因小鼠纹状体中多巴胺生物合成的调节机制”Neurosci.Lett。
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