Genetic map of the region around grizzled (gr) and mocha (mh) on mouse chromosome 10, homologous to human 19p13.3.
Genetic map of the region around grizzled (gr) and mocha (mh) on mouse chromosome 10, homologous to human 19p13.3.
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小鼠 10 号染色体上灰色 (gr) 和摩卡 (mh) 周围区域的遗传图谱，与人类 19p13.3 同源。
DOI：
10.1006/geno.1994.1552
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Burmeister,M
中科院分区：
文献类型：
--
作者：
Kapfhamer,D;Burmeister,M
灰色（gr）是一种隐性突变小鼠，导致灰色毛色和降低围产期生存能力。摩卡（mh）是几种隐性突变小鼠之一，其特征是血小板储存池障碍，色素异常，生育能力下降，肾功能缺陷，在某些突变体中，内耳和自然杀伤细胞缺陷。小鼠血小板储存池缺陷突变体可能是人类Chediak-Higashi和Hermansky-Pudlak综合征的模型。它们之间的基因连锁非常紧密（0 ± 1.2cM）。然而，它们相对于分子标记的相对位置以前是未知的。因此，对基因座的遗传作图也将产生关于它们的位置的信息。为了对这两个基因进行遗传学定位，我们对灰鼠和栗小家鼠进行了亚种间回交。在检测的539个后代中，我们没有发现gr基因、抗Muellerian激素（Amh）基因和微卫星标记D10 Mit 7、D10 Mit 21和D10 Mit 23之间的重组。每个侧翼标记Basigin（Bsg）和D10 Mit 22鉴定出一个重组事件。这些紧密连锁的标记应该为定位克隆外基因提供切入点。与灰熊相连的区域与人类染色体19p13.3上的基因丰富区域同源。
Grizzled(gr) is a recessive mouse mutation resulting in a gray coat color and reduced perinatal viability.Mocha(mh) is one of several recessive mouse mutants characterized by platelet storage pool disorder, pigment abnormalities, reduced fertility, kidney function deficiencies, and, in some mutants, inner ear and natural killer cell deficiencies. Murine platelet storage pool deficient mutants may be models for Chediak-Higashi and Hermansky-Pudlak syndromes in humans. The genes forgrandmhare very closely linked to each other (0 ± 1.2 cM). However, their relative position with respect to molecular markers was previously unknown. Thus, genetic mapping of thegrlocus will also yield information about themhlocation. To map these two genes genetically, we have performed an intersubspecific backcross ofgrizzledmice withMus musculus castaneus. In 539 progeny tested, we found no recombination between thegrgene, the gene for anti-Muellerian hormone (Amh), and the microsatellite markersD10Mit7, D10Mit21, andD10Mit23. One recombination event for each of the flanking markers Basigin (Bsg) andD10Mit22was identified. These closely linked markers should provide entry points for positional cloning of thegrandmhgenes. The region linked togrizzledis homologous to a gene-rich region on human Chromosome 19p13.3.