Ureibacillus composti sp nov and Ureibacillus thermophilus sp nov., isolated from livestock-manure composts
Ureibacillus composti sp nov and Ureibacillus thermophilus sp nov., isolated from livestock-manure composts
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DOI：
10.1099/ijs.0.65232-0
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发表时间：
2007-12-01
影响因子：
2.8
通讯作者：
Kwon, Soon-Wo
中科院分区：
文献类型：
--
作者：
Weon, Hang-Yeon;Lee, Seon-Young;Kwon, Soon-Wo
从韩国二村堆肥设施的堆肥样品中分离出两种革兰氏阴性杆状嗜热细菌菌株 HC145(T) 和 HC148(T)。对HC145(T)和HC148(T)的16S rRNA基因进行测序并对所得序列进行比较分析清楚地表明这些菌株与脲芽孢杆菌属具有系统发育关系。两个新菌株的16S rRNA相似度为98.4%，与现有脲芽孢杆菌的序列相似度为97.8-98.1(HC145(T))和97.4-98.7%(HC148(T))。两个菌株与 Ureibacillus 物种的模式菌株之间的 DNA-DNA 重关联值范围为 38% 至 51%。两种分离株的极性脂质谱均由组成未知的磷脂酰甘油、二磷脂酰甘油、磷脂和糖脂组成。主要醌类为MK-8、MK-9和MK-7，肽聚糖类型为L-Lys
Two Gram-negative, rod-shaped, thermophilic bacterial strains, HC145(T) and HC148(T), were isolated from a compost sample from a compost facility in Ichon, Korea. Sequencing of the 16S rRNA genes of HC145(T) and HC148(T) and comparative analyses of the resulting sequences clearly showed that these strains had a phylogenetic affiliation to the genus Ureibacillus. The level of 16S rRNA similarity between the two novel strains was 98.4 % and the levels of sequence similarity between them and existing Ureibacillus species were 97.8-98.1 (HC145(T)) and 97.4-98.7% (HC148(T)). The DNA-DNA reassociation values between the two strains and the type strains of Ureibacillus species ranged from 38 to 51 %. The polar lipid profiles for both isolates consisted of phosphatidylglycerol, diphosphatidylglycerol, phospholipids and glycolipids of unknown composition. The major quinones were MK-8, MK-9 and MK-7, the peptidoglycan type was L-Lys