Small angle neutron scattering of native and reconstituted silk fibroin
Small angle neutron scattering of native and reconstituted silk fibroin
复制标题
DOI：
10.1039/c0sm00108b
复制
发表时间：
2010-01-01
期刊：
影响因子：
3.4
通讯作者：
Vollrath, Fritz
中科院分区：
文献类型：
--
作者：
Greving, Imke;Dicko, Cedric;Vollrath, Fritz
蜘蛛和昆虫的丝是独立进化的蛋白质生物材料，具有迷人的物理特性，吸引了来自各个学科的科学家使用各种分析工具。在这里，我们证明了中子小角散射（SANS）在近体条件下研究天然丝素蛋白形态和结构的适用性。通过比较天然蚕丝和重组蚕丝的大小、分子量、再折叠和相互作用，我们发现了显著的差异。这些观察结果质疑了目前广泛应用于蚕丝分析的方法的有效性，即研究重组蚕丝的目的是获得有关天然蚕丝形成（储存和纺丝）机制的重要见解。
Silks spun by spiders and insects are independently evolved proteinaceous biomaterials with fascinating physical properties attracting scientists from a wide range of disciplines using a wide range of analytical tools. Here we demonstrate the suitability of neutron small angle scattering (SANS) to investigate the morphology and structure of native silk fibroin at near in vivo conditions. Comparing native and reconstituted silk we observed significant differences in sizes, molecular weights, refolding and interactions. These observations question the validity of a presently widespread approach in silk analysis i.e. studying reconstituted silk with the goal to gain important insights into the mechanisms involved in the formation (storage and spinning) of native silks.