A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae.
A Brazilian Marseillevirus Is the Founding Member of a Lineage in Family Marseilleviridae.
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DOI：
10.3390/v8030076
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发表时间：
2016-03-10
期刊：
影响因子：
--
通讯作者：
La Scola B
中科院分区：
文献类型：
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作者：
Dornas FP;Assis FL;Aherfi S;Arantes T;Abrahão JS;Colson P;La Scola B
2003年，多食棘阿米巴模拟病毒(APMV)被发现寄生在棘阿米巴上。发现其具有显著的特征，特别是奇特的基因组特征，并建立了病毒家族米米病毒科。随后，第二个巨型阿米巴病毒家族被描述，马赛病毒科，其原型成员是马赛病毒，于2009年被发现。目前，该家族七个不同成员的基因组已经完全测序。以往的系统发育分析表明，存在A、B和C三个马赛病毒科，这里我们描述了这个家族的一个新成员-巴西马赛病毒(BrMV)，它是从巴西的一个样本中分离出来的，其基因组已经被全序列测定和分析。令人惊讶的是，来自系统发育分析和比较基因组学的数据，包括BrMV与马赛病毒科其他成员的平均氨基酸同源性，以及对马赛病毒核心基因组和泛基因组的分析，表明该病毒可归属于一个新的马赛病毒科。尽管BrMV基因组是马赛病毒科成员中最小的之一，但它拥有这个家族第二大的基因含量。此外，BrMV基因组编码29个ORFan。在这里，我们描述了BrMV毒株的分离和基因组分析，并建议将其归类为马赛病毒科一个新的D谱系的原型病毒。
In 2003, Acanthamoeba polyphaga mimivirus (APMV) was discovered as parasitizing Acanthamoeba. It was revealed to exhibit remarkable features, especially odd genomic characteristics, and founded viral family Mimiviridae. Subsequently, a second family of giant amoebal viruses was described, Marseilleviridae, whose prototype member is Marseillevirus, discovered in 2009. Currently, the genomes of seven different members of this family have been fully sequenced. Previous phylogenetic analysis suggested the existence of three Marseilleviridae lineages: A, B and C. Here, we describe a new member of this family, Brazilian Marseillevirus (BrMV), which was isolated from a Brazilian sample and whose genome was fully sequenced and analyzed. Surprisingly, data from phylogenetic analyses and comparative genomics, including mean amino acid identity between BrMV and other Marseilleviridae members and the analyses of the core genome and pan-genome of marseilleviruses, indicated that this virus can be assigned to a new Marseilleviridae lineage. Even if the BrMV genome is one of the smallest among Marseilleviridae members, it harbors the second largest gene content into this family. In addition, the BrMV genome encodes 29 ORFans. Here, we describe the isolation and genome analyses of the BrMV strain, and propose its classification as the prototype virus of a new lineage D within the family Marseilleviridae.