Effects of L-arabinose by hypoglycemic and modulating gut microbiome in a high-fat diet- and streptozotocin-induced mouse model of type 2 diabetes mellitus.
Effects of L-arabinose by hypoglycemic and modulating gut microbiome in a high-fat diet- and streptozotocin-induced mouse model of type 2 diabetes mellitus.
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DOI：
10.1111/jfbc.13991
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发表时间：
2021-11
影响因子：
4
通讯作者：
Dan Shen;Yingjian Lu;Shuhua Tian;Shaotong Ma;Jing Sun;Qiaobin Hu;Xinyi Pang;Xiangfei Li
中科院分区：
文献类型：
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作者：
Dan Shen;Yingjian Lu;Shuhua Tian;Shaotong Ma;Jing Sun;Qiaobin Hu;Xinyi Pang;Xiangfei Li
L-阿拉伯糖是一种良好的健康食品添加剂。本研究旨在探讨 L-阿拉伯糖对高脂饮食 (HFD) 和链脲佐菌素 (STZ) 诱导的 2 型糖尿病 (T2DM) 小鼠模型的影响。模型小鼠通过口服管饲法接受20和60 mg（kg体重[bw]）-1 d-1的L-阿拉伯糖、300 mg（kg bw）-1 d-1的二甲双胍（阳性对照）或无菌水（对照）。与模型组相比，L-阿拉伯糖治疗的小鼠表现出糖尿病症状减轻，包括体重减轻速度较慢、β细胞功能指数水平的稳态模型评估增加、血糖降低、脂肪变性减轻和胰岛细胞修复。 L-阿拉伯糖还具有抗炎作用并部分减轻血脂异常。对肠道微生物群的 16S-rRNA 序列分析表明，在门水平上，L-阿拉伯糖处理显着降低了厚壁菌门与拟杆菌门的比例，因为厚壁菌门的相对丰度降低；在属水平上，它逆转了因暴露于 HFD 和 STZ 导致的 Allobaculum 相对丰度的增加和 Oscillospira 丰度的减少。并且模型小鼠给予20 mg(kg bw)-1 d-1 L-阿拉伯糖对改善T2DM的效果优于补充60 mg(kg bw)-1 d-1 L-阿拉伯糖高剂量组。这些结果强烈表明 L-阿拉伯糖是预防或治疗 T2DM 的绝佳候选补充剂。实际应用：L-阿拉伯糖、木糖醇和三氯蔗糖是众所周知的蔗糖替代品。据报道，L-阿拉伯糖对高血糖、血糖指数和脂肪积累具有有益作用。在这项研究中，我们发现低剂量（20 mg（kg bw）-1 d-1）补充L-阿拉伯糖可显着改善由 HFD 和 STZ 引起的 T2DM 的葡萄糖耐受不良和肠道菌群失调。
L-arabinose is a good and healthy food additive. This study was conducted to investigate the effect of L-arabinose in a mouse model of type 2 diabetes mellitus (T2DM) induced by exposure to a high-fat diet (HFD) and streptozotocin (STZ). The model mice received L-arabinose at 20 and 60 mg (kg body weight [bw])-1 d-1 , metformin at 300 mg (kg bw)-1 d-1 (positive control) or sterile water (control) via oral gavage. Compared with the model group, mice treated with L-arabinose exhibited attenuated symptoms of diabetes mellitus, including a slower rate of body weight loss, increased homeostasis model assessment of β-cell function index levels, decreased blood glucose, alleviation of steatosis, and repair of pancreatic islet cells. L-arabinose also exerted an anti-inflammatory effect and partially mitigated dyslipidemia. A 16S-rRNA sequence analysis of the gut microbiota revealed that at the phylum level, treatment with L-arabinose significantly reduced the ratio of Firmicutes to Bacteroidetes due to a decreased relative abundance of Firmicutes; at the genus level, it reversed the increase in the relative abundance of Allobaculum and the decrease abundance of Oscillospira caused by exposure to an HFD and STZ. And the model mice received L-arabinose at 20 mg (kg bw)-1 d-1 had a better effect on improving T2DM than the high-dose group supplemented L-arabinose at 60 mg (kg bw)-1 d-1 . These results strongly suggest L-arabinose as an excellent candidate supplement to prevent or treat T2DM. PRACTICAL APPLICATIONS: L-arabinose, xylitol and sucralose are well-known substitutes for sucrose. L-arabinose has been reported to have beneficial effects on hyperglycemia, glycemic index, and fat accumulation. In this study, we found that low-dose (20 mg (kg bw)-1 d-1 ) supplementation of L-arabinose significantly improved glucose intolerance and gut microbiota incoordination in T2DM caused by HFD and STZ.