Hedgehog targets in the Drosophila embryo and the mechanisms that generate tissue-specific outputs of Hedgehog signaling
Hedgehog targets in the Drosophila embryo and the mechanisms that generate tissue-specific outputs of Hedgehog signaling
复制标题
DOI：
10.1242/dev.055871
复制
发表时间：
2010-11-15
期刊：
影响因子：
4.6
通讯作者：
Kornberg, Thomas B.
中科院分区：
文献类型：
--
作者：
Biehs, Brian;Kechris, Katerina;Kornberg, Thomas B.
旁分泌刺猬（Hh）信号调节许多果蝇器官的生长和模式。我们绘制了Cubitus interruptus（Ci）的染色质结合位点，Cubitus interruptus是介导Hh信号转导输出的转录因子，我们分析了对照和突变胚胎的转录谱，以确定受Hh调控的基因。我们确定的推定目标包括几个Hh途径的组成部分，主要是以前确定的目标，和许多新的目标。我们分析的每一个Hh目标，不是一个通路组件似乎是由Hh在组织特异性的方式进行调节;通路组件和靶基因的表达模式的分析提供了证据的自分泌Hh信号在视原基的胚胎。我们提出的证据表明，组织特异性的Hh目标依赖于转录因子，是Hh独立的，这表明“前模式”的转录因子的合作伙伴与Ci，使Hh依赖的基因表达的位置特异性。
Paracrine Hedgehog (Hh) signaling regulates growth and patterning in many Drosophila organs. We mapped chromatin binding sites for Cubitus interruptus (Ci), the transcription factor that mediates outputs of Hh signal transduction, and we analyzed transcription profiles of control and mutant embryos to identify genes that are regulated by Hh. Putative targets that we identified included several Hh pathway components, mostly previously identified targets, and many targets that are novel. Every Hh target we analyzed that is not a pathway component appeared to be regulated by Hh in a tissue-specific manner; analysis of expression patterns of pathway components and target genes provided evidence of autocrine Hh signaling in the optic primordium of the embryo. We present evidence that tissue specificity of Hh targets depends on transcription factors that are Hh-independent, suggesting that 'pre-patterns' of transcription factors partner with Ci to make Hh-dependent gene expression position specific.