Anonymous single-copy nuclear DNA (scnDNA) markers for Grey-cheeked Fulvetta (Alcippe morrisonia) and Rufous-capped Babbler (Stachyridopsis ruficeps)
Anonymous single-copy nuclear DNA (scnDNA) markers for Grey-cheeked Fulvetta (Alcippe morrisonia) and Rufous-capped Babbler (Stachyridopsis ruficeps)
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灰颊鹛 (Alcippe morrisonia) 和红帽鹛 (Stachyridopsis ruficeps) 的匿名单拷贝核 DNA (scnDNA) 标记
DOI：
10.1007/s12686-012-9641-6
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发表时间：
2012-09-01
影响因子：
1.1
通讯作者：
Lei, Fumin
中科院分区：
文献类型：
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作者：
Gao, Bin;Qu, Yanhua;Lei, Fumin
我们报道了15个单拷贝匿名核基因座在斑嘴鸟Fulvetta(Alcippe Morrisonia)和11个红冠圆嘴蝠(Stachyridopsis Rufiep)中的发展。在桑天牛中检测了66个潜在基因座，在皱纹夜蛾中检测了65个潜在基因座。26个基因座的序列显示出高频率的单核苷酸多态。平均每千个碱基分别检测到39个和24个SNP(每个个体测序的总长度分别为12,063和12,296个碱基)。利用筛选条件对三个物种(Spizixos Semitorque、Parus Majus和Cyanopica cyana)的所有基因座进行跨物种扩增。这些标记为雀形目鸟类的生态、进化和保护研究提供了有用的资源。
We report the development of 15 single-copy anonymous nuclear loci for the babbler bird Grey-cheeked Fulvetta (Alcippe morrisonia) and 11 for Rufous-capped Babbler (Stachyridopsis ruficeps). 66 potential loci were tested in A. morrisonia and 65 potential loci were tested in S. ruficeps. Sequences of the 26 loci revealed a high frequency of single nucleotide polymorphisms. An average of 39 and 24 SNPs per kilobase were identified (total of 12,063 bp and 12,296 bp sequenced per individual) respectively. Cross-species amplification was achieved using screening conditions as for all loci in three species (Spizixos semitorques, Parus major and Cyanopica cyana). The markers documented here provide a useful resource for ecology, evolution and conservation research of passerine birds.