Changes in vitellin and other yolk proteins during embryonic development in the silkworm, Bombyx mori
Changes in vitellin and other yolk proteins during embryonic development in the silkworm, Bombyx mori
复制标题
DOI：
10.1016/0022-1910(80)90097-9
复制
发表时间：
1980
影响因子：
2.2
通讯作者：
Kenichi Irie;O. Yamashita
中科院分区：
文献类型：
--
作者：
Kenichi Irie;O. Yamashita
用聚丙烯酰胺凝胶电泳和免疫滴定技术研究了家蚕卵在胚胎发育过程中卵黄蛋白和其他卵黄蛋白的含量变化。在新产的鸡蛋中，可溶性蛋白质经电泳分离成至少9条带。经鉴定，主要条带为卵黄蛋白，约占总蛋白的40%。除卵黄蛋白等4条优势带为卵特异带外，其余4条优势带的迁移率与血淋巴蛋白相同，约占总蛋白的20%，在产卵后6-7天的胚胎分化期，总蛋白含量没有下降，带型和相对浓度基本不变。然而，蛋特异性蛋白质的浓度稳步下降。在随后的幼虫分化至孵化期间，总蛋白质的利用率约为初始水平的50%，几乎所有种类的蛋白质都发生了快速降解，免疫滴定实验进一步证明卵黄蛋白在胚胎分化期间不被利用，但在幼虫分化期间被显著消耗。但在初孵仔鱼中保留了约30%的初始水平。这种持久性卵黄蛋白的讨论有关的蛋白质代谢过程中胚胎发育在蚕。
Changes in the amounts of vitellin and other yolk proteins of the eggs of the silkworm,Bombyx moriwere investigated during embryonic development using polyacrylamide gel electrophoresis and immunotitration techniques. In the newly laid eggs, soluble proteins were separated into at least nine bands after electrophoresis. The major band was identified as vitellin, accounting for about 40% of the total proteins. The four predominant bands including vitellin exhibited the same mobility as the proteins of haemolymph, but one other major band was specific to the eggs, accounting for about 20% of the proteins.During embryonic differentiation 6–7 days after oviposition, the total protein content did not decrease and the banding patterns and their relative concentrations remained unchanged as a whole. However the concentration of the egg specific protein steadily decreased. During subsequent larval differentiation until hatching, the total proteins were utilized to about 50% of the initial levels: the rapid degradation was observed in almost every species of proteins.An immunotitration experiment further demonstrated that vitellin was not utlilized during embryonic differentiation but was consumed markedly during larval differentiation. However, about 30% of initial level was reserved in the newly hatched larvae. Such a prolonged persistence of vitellin is discussed in relation to protein metabolism during embryonic development in silkworms.