Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin
Expression of interleukin-8 in primary and metastatic malignant melanoma of the skin
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DOI：
10.1097/00008390-199908000-00007
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发表时间：
1999-08-01
影响因子：
2.2
通讯作者：
Johansson, SL
中科院分区：
文献类型：
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作者：
Singh, RK;Varney, ML;Johansson, SL
最近的研究表明，白细胞介素-8 （IL-8）是由培养的黑色素瘤细胞产生的，并作为一种必需的自分泌生长因子。我们实验室早期的研究表明，IL-8在人类变异细胞系中的表达与其在裸鼠中的转移潜能之间存在直接相关性。在本研究中，我们使用免疫组织化学方法检测了人类恶性黑色素瘤中IL-8的表达，以确定IL-8水平与疾病分期的相关性。桡骨生长期（RGP）肿瘤（原位黑色素瘤）均无IL-8表达。相反，50%具有高转移风险的垂直生长期（VGP）肿瘤（侵袭性黑色素瘤）显示IL-8免疫反应性。此外，分析的所有转移性病灶均显示IL-8高水平染色；总的来说，这些数据表明IL-8的表达与转移表型之间存在关联。(C) 1999 Lippincott Williams & Wilkins。
It was recently demonstrated that interleukin-8 (IL-8) is produced by cultured melanoma cells and acts as an essential autocrine growth factor. Earlier studies from our laboratory demonstrated a direct correlation between IL-8 expression in human variant cell lines and their metastatic potential in nude mice. In the present study we examined the expression of IL-8 in human malignant melanomas using immunohistochemistry to correlate IL-8 levels with disease stage. None of the radial growth phase (RGP) tumours (melanoma in situ) expressed IL-8. In contrast, 50% of the vertical growth phase (VGP) tumours (invasive melanoma), which have a high risk of metastasis, showed IL-8 immunoreactivity. Further, all the metastatic lesions analysed showed intense staining for IL-8; the levels were higher than those observed in the primary skin tumours, In summary, the data suggest an association between the expression of IL-8 and the metastatic phenotype. (C) 1999 Lippincott Williams & Wilkins.