Design and Development of Enzymatically Activatable Photosensitizer Based on Unique Characteristics of Thiazole Orange
Design and Development of Enzymatically Activatable Photosensitizer Based on Unique Characteristics of Thiazole Orange
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DOI：
10.1021/ja900443b
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发表时间：
2009-05-06
影响因子：
15
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
--
作者：
Koide, Yuichiro;Urano, Yasuteru;Nagano, Tetsuo
开发了一种控制噻唑橙（TO）荧光和光敏能力的新颖设计策略。该方法的有效性通过 B-半乳糖苷酶激活光敏剂 PhoTO-Gal 的合成得到了证明，其中荧光同时被激活。在光照下，PhoTO-Gal 被证明可以杀死表达 β-半乳糖苷酶的 HEK293 lacZ(+) 细胞，但不能杀死 HEK293 lacZ(-) 细胞。这种可激活的光敏剂应该允许更精细的PDT，而不会产生长期严格敏感性的副作用，并且还应该可用作报告酶表达特异性细胞消融的工具。
A novel design strategy for controlling the fluorescence and photosensitizing ability of thiazole orange (TO) has been developed. The validity of this approach was demonstrated by the synthesis of a B-galactosidase-activatable photosensitizer, PhoTO-Gal, in which fluorescence is simultaneously activated. PhoTO-Gal was demonstrated to kill HEK293 lacZ(+) cells, which express beta-galactosidase, but not HEK293 lacZ(-) cells, under light illumination. Such activatable photosensitizers should allow more refined PDT without the side effect of prolonged tight sensitivity and should also be useful as toots for reporter enzyme expression-specific cell ablation.