Differences between blood cells of juvenile and adult specimens of the pond snail Lymnaea stagnalis
Differences between blood cells of juvenile and adult specimens of the pond snail Lymnaea stagnalis
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DOI：
10.1007/bf00215142
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发表时间：
1984-09
影响因子：
3.6
通讯作者：
R. Dikkeboom;W. Knaap;E. A. Meuleman;T. Sminia
中科院分区：
文献类型：
--
作者：
R. Dikkeboom;W. Knaap;E. A. Meuleman;T. Sminia
比较了滞流池螺幼螺和成螺的血细胞（变形细胞）。幼螺每μl血淋巴中含有较少的循环变形细胞。然而，较高比例的这些细胞显示有丝分裂活性，通过掺入3 H-胸苷在体外测定。相对较多的幼螺变形细胞具有分化程度较低的细胞特征：它们小而圆，内含物较少，核质比高，派洛宁含量高。酶细胞化学研究表明，酸性磷酸酶（AcP），非特异性酯酶（NSE），碱性磷酸酶（AlP）存在于所有的阿米巴细胞的少年和成年蜗牛。AcP活性相对较弱。NSE活性分散在整个细胞质中，偶尔在颗粒中发现，而AlP明显定位于颗粒中。两个年龄组之间的差异，发现只有酶过氧化物酶（PO）。在少年蜗牛的细胞的百分比较低是积极的，含有活性的颗粒是不丰富的比成人的变形细胞。作者认为，由于变形细胞的上述特性，幼年鼠体内防御系统的活性可能与其体内的细胞分裂有关。stagnalisis在一个较低的水平比成年蜗牛。这可能是一个解释的事实，青少年。滞育螺对眼斑毛毕吸虫感染高度敏感，而成年螺则不太敏感。
Blood cells (amoebocytes) of juvenile and adult specimens of the pond snailLymnaea stagnaliswere compared. Juvenile snails contain fewer circulating amoebocytes per μl haemolymph. However, a higher percentage of these cells shows mitotic activity, as determined by incorporation of3H-thymidine in vitro. Relatively more amoebocytes of juvenile snails have the characteristics of less differentiated cells: they are small and round with few inclusions, a high nucleus-to-cytoplasm ratio, and a high pyronin stainability. Enzyme cytochemical studies showed that acid phosphatase (AcP), non-specific esterase (NSE), and alkaline phosphatase (AlP) are present in all amoebocytes of juvenile and adult snails. AcP activity is relatively weak. NSE activity is dispersed throughout the cytoplasm and occasionally found in granules, whereas AlP is clearly localized in granules. Differences between the two age groups were found only for the enzyme peroxidase (PO). In juvenile snails a lower percentage of the cells is positive and the granules that contain the activity are less abundant than in amoebocytes of adults. It is suggested that, due to the above-mentioned characteristics of the amoebocytes, the activity of the internal defence system in juvenileL. stagnalisis on a lower level than that in adult snails. This might be an explanation for the fact that juvenileL. stagnalisare highly susceptible to infection by the schistosomeTrichobilharzia ocellata, whereas adult snails are less susceptible.