Do proteins denature during droplet evolution in electrospray ionization?
Do proteins denature during droplet evolution in electrospray ionization?
复制标题
DOI：
10.1016/s0168-1176(96)04517-x
复制
发表时间：
1997-03-01
期刊：
影响因子：
--
通讯作者：
Chait, BT
中科院分区：
文献类型：
--
作者：
Mirza, UA;Chait, BT
本文所描述的实验旨在解决蛋白质是否在电喷雾电离相变的毫秒时间尺度上发生变性的问题。实验表明，这是相对困难的诱导电荷的变化，在选定的蛋白质的电喷雾液滴从大气压到真空的质谱仪的转移过程中，通过施加热量。这些发现表明，由于加热管中液滴的寿命短（0.6 ms或更短），液滴的净传热不足以变性，并提供了部分解释，为什么溶液中平衡蛋白质的电荷分布特征在一定程度上保留在气相中。我们的研究结果使我们提出了一般的指导方针，以改善有关溶液蛋白质构象和非共价缔合通过电喷雾电离质谱测量获得的信息。(C)1997年Elsevier Science B.V.
The experiments described in this paper were designed to address the question of whether proteins undergo denaturation on the millisecond timescale of the phase transition in electrospray ionization. The experiments show that it is relatively difficult to induce a change in charge in selected proteins by the application of heat to electrosprayed droplets during their transfer from atmospheric pressure to the vacuum of the mass spectrometer. These findings indicate that the net heat transfer to the droplets is insufficient for denaturation given the short lifetime of the droplets in the heated tube (0.6 ms or less) and provide a partial explanation as to why the charge distribution characteristic of a protein in equilibrium in solution is to some extent preserved into the gas phase. Our findings lead us to suggest general guidelines for improving the information about solution protein conformation and non-covalent associations obtained by electrospray ionization mass spectrometric measurements. (C) 1997 Elsevier Science B.V.