Relationships between subgingival microbiota and GCF biomarkers in generalized aggressive periodontitis.
Relationships between subgingival microbiota and GCF biomarkers in generalized aggressive periodontitis.
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DOI：
10.1111/j.1600-051x.2010.01534.x
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发表时间：
2010-04
影响因子：
6.7
通讯作者：
Haffajee AD
中科院分区：
文献类型：
--
作者：
Teles RP;Gursky LC;Faveri M;Rosa EA;Teles FR;Feres M;Socransky SS;Haffajee AD
研究牙周健康和广泛性侵袭性牙周炎 (GAP) 中龈下生物膜组成与龈沟液 (GCF) 细胞因子水平之间的关系。对 25 名牙周健康受试者和 31 名 GAP 受试者进行了牙周参数测量。从每位受试者的 14 个部位获取龈下菌斑和 GCF 样本。使用棋盘 DNA-DNA 杂交对 40 个龈下类群进行定量，并使用 Luminex 测量 8 种 GCF 细胞因子的浓度。使用聚类分析来定义每个临床组中具有相似龈下微生物群的位点。使用 Kruskal-Wallis 检验确定簇间临床、微生物学和免疫学参数差异的显着性。与牙周健康受试者相比，GAP 受试者的白细胞介素 1β (IL-1β) (p<0.001)、粒细胞-巨噬细胞集落刺激因子 (GM-CSF) (p<0.01) 和 IL-1β/IL-10 比率 (p<0.001) 的 GCF 水平显着较高，并且红色和橙色复合物种类的比例较高。牙周健康受试者中簇间细胞因子的平均比例没有统计学上的显着差异，而侵袭性牙周炎组中簇间IL-1β/IL-10的比率（p<0.05）存在显着差异。不同的龈下生物膜特征与不同的 GCF 细胞因子表达模式相关。侵袭性牙周炎受试者的特点是 IL-1β/IL-10 比值高于牙周健康受试者，表明侵袭性牙周炎中促炎细胞因子和抗炎细胞因子之间存在不平衡。
To examine relationships between subgingival biofilm composition and levels of gingival crevicular fluid (GCF) cytokines in periodontal health and generalized aggressive periodontitis (GAP). Periodontal parameters were measured in 25 periodontally healthy and 31 GAP subjects. Subgingival plaque and GCF samples were obtained from 14 sites from each subject. 40 subgingival taxa were quantified using checkerboard DNA-DNA hybridization and the concentrations of 8 GCF cytokines measured using Luminex. Cluster analysis was used to define sites with similar subgingival microbiotas in each clinical group. Significance of differences in clinical, microbiological and immunological parameters among clusters was determined using the Kruskal-Wallis test. GAP subjects had statistically significantly higher GCF levels of interleukin-1β (IL-1β) (p<0.001), granulocyte-macrophage colony-stimulating factor (GM-CSF) (p<0.01), and IL-1β/IL-10 ratio (p<0.001) and higher proportions of Red and Orange complex species than periodontally healthy subjects. There were no statistically significant differences in the mean proportion of cytokines among clusters in the periodontally healthy subjects, while the ratio IL-1β/IL-10 (p<0.05) differed significantly among clusters in the aggressive periodontitis group. Different subgingival biofilm profiles are associated with distinct patterns of GCF cytokine expression. Aggressive periodontitis subjects were characterized by a higher IL-1β/IL-10 ratio than periodontally healthy subjects, suggesting an imbalance between pro- and anti-inflammatory cytokines in aggressive periodontitis.