Specific requirements for cytochrome c-550 and the manganese-stabilizing protein in photoautotrophic strains of Synechocystis sp. PCC 6803 with mutations in the domain Gly-351 to Thr-436 of the chlorophyll-binding protein CP47.
Specific requirements for cytochrome c-550 and the manganese-stabilizing protein in photoautotrophic strains of Synechocystis sp. PCC 6803 with mutations in the domain Gly-351 to Thr-436 of the chlorophyll-binding protein CP47.
复制标题
集胞藻光合自养菌株对细胞色素 c-550 和锰稳定蛋白的具体要求。
DOI：
10.1021/bi980404s
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
J. Eaton‐Rye
中科院分区：
文献类型：
--
作者：
Trefor Owen Morgan;J. A. Shand;S. Clarke;J. Eaton‐Rye
已在集胞藻中评估了光系统 II (PSII) 中细胞色素 c-550 (PSII-V) 的需求。 PCC 6803 含有叶绿素 a 结合蛋白 CP47 环 E 结构域的 Gly-351 和 Thr-436 之间的突变。利用六种光合自养菌株来比较去除锰稳定蛋白 (PSII-O) 或 PSII-V 对体内 PSII 活性的影响。这些是野生型对照；两个具有氨基酸缺失的菌株，Delta(R384-V392)和Delta(G429-T436)；三个携带特定氨基酸取代，G351L/T365Q、G351L/E364Q/T365Q 和 G351L/E353Q/E355Q/T365Q。 PSII-O的去除阻止了PSII在Delta(G429-T436)中的组装，但在Delta(R384-V392)中则不然。在没有 PSII-V 的情况下，Delta(G429-T436) 和 Delta(R384-V392) 都不能支持光合自养生长。在氯限制条件下，Delta(R384-V392)的光自养生长受到严重损害，Delta(G429-T436)的光合自养生长完全受到抑制，并且缺乏PSII-V的菌株不能在氯限制或钙限制培养基中生长。 Gly-351、Glu-353、Glu-355 和 Thr-365 的取代产生的表型与存在或不存在 PSII-O 和 PSII-V 的对照相似，但从 G351L/E364Q/T365Q 中去除 PSII-O 会导致组装的 PSII 中心显着减少，并增强对光失活的敏感性，同时去除 PSII-V 会阻止光合自养成长。其他突变体 E364Q:DeltaPSII-V 和 E364G:DeltaPSII-V 证明这种抑制是 Glu-364 突变的结果。这些结果还表明，体内去除 PSII-V 在所检测的 CP47 突变体中产生的表型与去除 PSII-O 产生的表型相似或更严重。
The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either the manganese-stabilizing protein (PSII-O) or PSII-V on PSII activity in vivo. These were a wild-type control; two strains with amino acid deletions, Delta(R384-V392) and Delta(G429-T436); and three carrying specific amino acid substitutions, G351L/T365Q, G351L/E364Q/T365Q, and G351L/E353Q/E355Q/T365Q. The removal of PSII-O prevented the assembly of PSII in Delta(G429-T436) but not in Delta(R384-V392). Neither Delta(G429-T436) nor Delta(R384-V392) could support photoautotrophic growth in the absence of PSII-V. In chloride-limiting conditions, the photoautotrophic growth of Delta(R384-V392) was severely impaired and that of Delta(G429-T436) totally inhibited, and no strains lacking PSII-V could grow in chloride-limiting or calcium-limiting media. Substitutions at Gly-351, Glu-353, Glu-355, and Thr-365 produced phenotypes that were similar to those of the control in the presence or absence of PSII-O and PSII-V, but removal of PSII-O from G351L/E364Q/T365Q produced a significant reduction of assembled PSII centers and an enhanced sensitivity to photoinactivation while removal of PSII-V prevented photoautotrophic growth. The additional mutants E364Q:DeltaPSII-V and E364G:DeltaPSII-V demonstrated that this inhibition was a consequence of the mutation at Glu-364. These results also show that the removal of PSII-V, in vivo, produces phenotypes in the CP47 mutants examined that are either similar or more severe than those resulting from the removal of PSII-O.