Bacterial Derived Carbohydrates Bind Cyrl and Trigger Hyphal Growth in Candida albicans
Bacterial Derived Carbohydrates Bind Cyrl and Trigger Hyphal Growth in Candida albicans
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DOI：
10.1021/acsinfecdis.7b00154
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发表时间：
2018-01-01
影响因子：
5.3
通讯作者：
Grimes, Catherine L.
中科院分区：
文献类型：
--
作者：
Burch, Jason M.;Mashayekh, Siavash;Grimes, Catherine L.
双相酵母菌白色念珠菌是人类最常见的致病真菌。虽然该物种通常是共生的，但从芽殖酵母到丝状菌丝的形态转变使真菌能够侵入上皮细胞并引起感染。表型变化由腺苷酸环化酶Cyrl控制。有趣的是，这种蛋白质含有一个富含亮氨酸的重复序列（LRR）结构域，它通常存在于植物和动物的先天免疫受体中。从E. coli表达。利用表面等离子体共振测定，发现LRR以高亲和力结合多种细菌衍生的碳水化合物。该结构域能够结合肽聚糖的片段，肽聚糖是细菌细胞壁的碳水化合物聚合物组分，以及链霉菌产生的蒽环类抗生素，导致菌丝形成。这些发现为人类微生物组增加了另一个维度，考虑到宿主中发生的酵母菌相互作用。
The dimorphic yeast Candida albicans is the most common pathogenic fungus found in humans. While this species is normally commensal, a morphological switch from budding yeast to filamentous hyphae allows the fungi to invade epithelial cells and cause infections. The phenotypic change is controlled by the adenylyl cyclase, Cyrl. Interestingly, this protein contains a leucine-rich repeat (LRR) domain, which is commonly found in innate immune receptors from plants and animals. A functional and pure LRR domain was obtained in high yields from E. coli expression. Utilizing a surface plasmon resonance assay, the LRR was found to bind diverse bacterial derived carbohydrates with high affinity. This domain is capable of binding fragments of peptidoglycan, a carbohydrate polymer component of the bacterial cell wall, as well as anthracyclines produced by Streptomyces, leading to hyphae formation. These findings add another dimension to the human microbiome, taking into account yeast bacteria interactions that occur in the host.