Expression of cytoskeleton regulatory protein Mena in human hepatocellular carcinoma and its prognostic significance
Expression of cytoskeleton regulatory protein Mena in human hepatocellular carcinoma and its prognostic significance
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DOI：
10.1007/s12032-014-0939-y
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发表时间：
2014-05-01
期刊：
影响因子：
3.4
通讯作者：
Xu, Lihua
中科院分区：
文献类型：
--
作者：
Hu, Kunpeng;Wang, Jiani;Xu, Lihua
肝细胞癌（HCC）发生发展的分子机制目前尚不清楚。本研究的主要目的是分析Enabled [哺乳动物Ena（Mena）]蛋白在人肝癌中的表达及其临床意义。采用实时定量PCR和Western blotting方法检测10例肝癌组织及癌旁正常肝组织中Mena的表达。采用免疫组化法检测81例HCC组织中Mena蛋白的表达。分析Mena表达与HCC临床病理特征的关系，并评估HCC患者的预后。结果显示：10对肝癌组织中Mena mRNA和蛋白的表达均高于癌旁正常组织。免疫组化染色显示，Mena主要积聚在肿瘤细胞胞浆中，40.74%（33/81）的患者Mena过表达。Mena过表达与肿瘤分化程度低（P = 0.025）、肿瘤分期晚（P = 0.003）和无病生存率低（DFS，P < 0.001）相关。此外，多因素分析中，Mena是DFS的独立预后因素（HR 2.309，95%CI 1.104-4.828; P = 0.026）. Mena在HCC中表达上调，并与肿瘤分化程度和临床分期相关。Mena可能是HCC患者DFS的独立预后指标。
The molecular mechanisms of the development and progression of hepatocellular carcinoma (HCC) are poorly understood. The main objective of this study was to analyze the expression of Enabled [mammalian Ena (Mena)] protein and its clinical significance in human HCC. The Mena expression was examined at mRNA and protein levels by real-time quantitative polymerase chain reaction and Western blotting analysis in ten paired HCC tissues and the adjacent normal tissues. The expression of Mena protein in 81 specimens of HCC tissues was determined by immunohistochemistry. Associations of Mena expression with the clinicopathological features were analyzed, and prognosis of HCC patients was evaluated. The result shows the expression of Mena mRNA and protein was higher in HCC than in the adjacent normal tissues in ten paired samples. Mena was mainly accumulated in the cytoplasm of tumor cells and over-expressed in 40.74 % (33/81) patients by immunohistochemical staining. Overexpression of Mena was significantly associated with poor cellular differentiation (P = 0.025), advanced tumor stage (P = 0.003) and worse disease-free survival (DFS, P < 0.001). In addition, Mena is an independent prognostic factor for DFS in multivariate analysis (HR 2.309, 95 % CI 1.104-4.828; P = 0.026). Mena is up-regulated in HCC and associated with tumor differentiation and clinical stage. Mena may be an independent prognostic marker for DFS of HCC patients.