Electrochemical interrogation of DNA monolayers on gold surfaces
Electrochemical interrogation of DNA monolayers on gold surfaces
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DOI：
10.1021/ac050911x
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发表时间：
2005-10-01
影响因子：
7.4
通讯作者：
Fan, CH
中科院分区：
文献类型：
--
作者：
Lao, RJ;Song, SP;Fan, CH
在本报告中，我们利用电化学技术系统地研究了金表面的 DNA 固定。比较循环伏安法和计时库仑法研究表明，固定在金表面的 DNA 单层不是均匀的。即使经过巯基苯沙醇处理，非特异性 Au-DNA 相互作用仍然存在，众所周知，巯基苯沙醇可以竞争性地去除松散的结合。金表面的 DNA。虽然硫醇化和非硫醇化 DNA 都在金表面形成单层，但它们的杂交能力明显不同。与硫醇化 DNA 探针相比，固定在金表面的非硫醇化 DNA 探针基本上是不可杂交的。这里提出的实验结果可能对高性能电化学 DNA 传感器的设计有用。
In this report, we systematically investigated DNA immobilization at gold surfaces with electrochemical techniques. Comparative cyclic voltammetric and chrono-coulometric studies suggested that DNA monolayers immobilized at gold surfaces were not homogeneous. Nonspecific Au-DNA interactions existed even with the treatment of mercaptobexanol, which was known to competitively remove loosely bound. DNA at gold surfaces. While both thiolated and nonthiolated DNA formed monolayers on gold surfaces, their hybridization abilities were distinctly different. In contrast to thiolated DNA probes, nonthiolated DNA probes immobilized at gold surfaces were essentially nonhybridizable. The experimental results presented here might be useful for the design of high-performance electrochemical DNA sensors.