Analysis on Actinobacillus pleuropneumoniae LuxS regulated genes reveals pleiotropic roles of LuxS/AI-2 on biofilm formation, adhesion ability and iron metabolism
Analysis on Actinobacillus pleuropneumoniae LuxS regulated genes reveals pleiotropic roles of LuxS/AI-2 on biofilm formation, adhesion ability and iron metabolism
复制标题
胸膜肺炎放线杆菌 LuxS 调控基因分析揭示 LuxS/AI-2 对生物膜形成、粘附能力和铁代谢的多效作用
DOI：
10.1016/j.micpath.2011.02.002
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发表时间：
2011-06-01
影响因子：
3.8
通讯作者：
Zhou, Rui
中科院分区：
文献类型：
--
作者：
Li, Lu;Xu, Zhuofei;Zhou, Rui
LuxS 是一种参与激活甲基循环的酶，副产物自诱导剂 2 (AI-2) 是某些物种中的群体感应信号。在我们之前的研究中，LuxS 在 AI-2 生产中的功能性在猪呼吸道病原体胸膜肺炎放线杆菌中得到了验证。在 luxS 突变体中观察到生物膜形成增强和毒力降低。为了全面了解luxS的功能，本研究利用微阵列技术比较了胸膜肺炎放线菌luxS突变体及其亲本菌株在四个不同生长期的转录谱。许多与感染相关的基因存在差异表达。 luxS突变体中生物膜形成基因pgaABC在指数早期上调，而与粘附相关的9个基因在指数后期下调。一组涉及铁获取和代谢的基因在四个生长阶段受到调节。使用 luxS 突变体以及对这些毒力性状的遗传和化学 (AI-2) 互补进行表型研究。结果表明，luxS突变体表现出增强的生物膜形成和降低的粘附能力，并且这些效应并不是由于缺乏AI-2所致。但 AI-2 可以增加胸膜肺炎放线菌的生物膜形成和粘附，与 LuxS 无关。 LuxS 可通过 AI-2 的产生来控制铁限制条件下的生长。这些结果揭示了 LuxS 和 AI-2 对胸膜肺炎放线菌毒力特征的多效性作用。 (C) 2011 Elsevier Ltd. 保留所有权利。
LuxS is an enzyme involved in the activated methyl cycle and the by-product autoinducer-2 (AI-2) was a quorum sensing signal in some species. In our previous study, the functional LuxS in AI-2 production was verified in the porcine respiratory pathogen Actinobacillus pleuropneumoniae. Enhanced biofilm formation and reduced virulence were observed in the luxS mutant. To comprehensively understand the luxS function, in this study, the transcriptional profiles were compared between the A. pleuropneumoniae luxS mutant and its parental strain in four different growth phases using microarray. Many genes associated with infection were differentially expressed. The biofilm formation genes pgaABC in the luxS mutant were up-regulated in early exponential phase, while 9 genes associated with adhesion were down-regulated in late exponential phase. A group of genes involved in iron acquisition and metabolism were regulated in four growth phases. Phenotypic investigations using luxS mutant and both genetic and chemical (AI-2) complementation on these virulence traits were performed. The results demonstrated that the luxS mutant showed enhanced biofilm formation and reduced adhesion ability and these effects were not due to lack of AI-2. But AI-2 could increase biofilm formation and adhesion of A. pleuropneumoniae independent of LuxS. Growth under iron restricted condition could be controlled by LuxS through AI-2 production. These results revealed pleiotropic roles of LuxS and AI-2 on A. pleuropneumoniae virulence traits. (C) 2011 Elsevier Ltd. All rights reserved.