Molecular cloning of the bovine CD9 antigen from ocular ciliary epithelial cells.
Molecular cloning of the bovine CD9 antigen from ocular ciliary epithelial cells.
复制标题
来自眼睫状上皮细胞的牛 CD9 抗原的分子克隆。
DOI：
10.1093/oxfordjournals.jbchem.a123866
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发表时间：
1992
影响因子：
2.7
通讯作者：
Coca-Prados,M
中科院分区：
文献类型：
--
作者：
Martín-Alonso,JM;Hernando,N;Ghosh,S;Coca-Prados,M
睫状上皮是一层双层的上皮细胞，负责哺乳动物眼睛房水的形成和分泌。我们从牛眼睫状体上皮细胞CD9GT11cDNA文库中分离到一个编码λ抗原的基因克隆，该基因是一个新的跨膜蛋白家族的成员。牛CD9克隆含有226个氨基酸(M-24,860)的开放阅读框。从牛CD9克隆推导的氨基酸序列与从巨核细胞中分离到的人CD9的氨基酸序列有83.5%的同源性，而与一组参与生长调控的相关抗原(TapA-1、CO-029、CD53、MRCox-44、ME491、CD63、CD37和Sm23)的同源性较低。对牛眼组织的分析表明，CD9基因编码1.4kb的mRNA，主要在角膜中检测到，在睫状体上皮、视网膜、虹膜和晶状体中有少量表达。从眼睫毛上皮建立的正常和转化的细胞系显示出显著的CD9转录水平。这些结果提出了关于CDO在眼前段可能扮演的角色的问题。
The ciliary epithelium is a bilayer of epithelial cells responsible for the formation and secretion of aqueous humor in the mammalian eye. We have isolated a cDNA clone from a λgtll cDNA library of bovine ocular ciliary epithelial cells encoding the CD9 antigen, a member of a new family of transmembrane proteins. The bovine CD9 clone contains an open reading frame of 226 amino acids (M- 24,860). The deduced amino acid sequence from the bovine CD9 cDNA clone shows 83.5% identity with the human counterpart isolated from megakaryocytes, and a lower degree of identity with a group of related antigens (TAPA-1, CO-029, CD53, MRC OX-44, ME491, CD63, CD37, and Sm23) involved in growth regulation. Analysis of bovine ocular tissues reveals that the CD9 gene encodes a 1.4 kb mRNA which is detectable predominantly in cornea and at low levels in ciliary epithelium, retina, iris, and lens. Normal and transformed cell lines established from the ocular ciliary epithelium exhibited significant levels of CD9 transcripts. These results raise questions regarding possible roles of CDO in the anterior segment of the eye.