Tolerance of arabinoxylan-oligosaccharides and their prebiotic activity in healthy subjects: a randomised, placebo-controlled cross-over study
Tolerance of arabinoxylan-oligosaccharides and their prebiotic activity in healthy subjects: a randomised, placebo-controlled cross-over study
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DOI：
10.1017/s0007114509992248
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发表时间：
2010-03-14
影响因子：
3.6
通讯作者：
Verbeke, Kristin
中科院分区：
文献类型：
--
作者：
Cloetens, Lieselotte;Broekaert, Willem F.;Verbeke, Kristin
研究了健康人口服由小麦纤维阿拉伯木聚糖部分酶解产生的阿拉伯低聚糖（AXOS）后的耐受性和益生元效应。共有20例健康受试者参加了本随机、安慰剂对照交叉研究。他们每天服用10 g AXOS或安慰剂，持续3周，中间有4周的洗脱期。在每个摄入期之前和之后立即采集血液样本以测量血液学和临床化学参数，并且受试者完成关于胃肠道症状的问卷。此外，在48小时内收集尿液用于通过GC-MS分析对甲酚和苯酚含量，并且在72小时内收集粪便用于使用实时PCR分析微生物群。在受试者中，10名受试者的尿液也在AXOS或安慰剂摄入量方面具有统计学显著性，但这些变化仍在正常范围内。血脂保持不变。AXOS对胃肠道症状的范围没有统计学显著影响，除了肠胃气胀轻微增加。服用AXOS两周后，尿中对甲酚排泄量（蛋白质发酵的一个指标）显著减少。在服用AXOS 2周和3周后以及服用安慰剂3周后，双歧杆菌的水平显著增加。然而，AXOS对双歧杆菌的影响比安慰剂更明显。总之，AXOS是一种耐受性良好的益生元，剂量为10 g/d。摄入AXOS可增加粪便中的双歧杆菌，减少尿中对甲酚的排泄。
The tolerance and prebiotic effect following oral intake by healthy human subjects of arabinoxylan-oligosaccharides (AXOS), produced by partial enzymic hydrolysis of the wheat fibre arabinoxylan, were studied. A total of twenty healthy subjects participated in the present randomised, placebo-controlled cross-over study. They consumed 10 g AXOS or placebo per d each for 3 weeks with a 4-week wash-out period in between. Before and immediately after each intake period, blood samples were taken to measure haematological and clinical chemistry parameters and the subjects completed a questionnaire about gastrointestinal symptoms. Additionally, urine was collected over 48 h for analysis of p-cresol and phenol content by GC-MS, and faeces were collected over 72 h for analysis of microbiota using real-time PCR. Of the subjects, ten also performed a urine statistically significantly way be either AXOS or placebo intake, but these changes remained within the normal range. Blood lipids remained unchanged. AXOS had no statistically significant effect on the range of gastrointestinal symptoms, except for a mild increase in flatulence. Urinary p-cresol excretion, an indicator of protein fermentation, was significantly decreased after 2 weeks of AXOS intake. The levels of bifidobacteria were significantly increased after 2 and 3 weeks of AXOS intake as well as after 3 weeks of placebo. However, the effect of AXOS on bifidobacteria was more pronounced than that of placebo. In conclusion, AXOS are a well-tolerated prebiotic at the dose of 10 g/d. AXOS intake increases faecal bifidobacteria and reduces urinary p-cresol excretion.