Next-generation DNA sequencing analysis of two Streptococcus suis ST28 isolates associated with human infective endocarditis and meningitis in Gunma, Japan: a case report
Next-generation DNA sequencing analysis of two Streptococcus suis ST28 isolates associated with human infective endocarditis and meningitis in Gunma, Japan: a case report
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DOI：
10.1080/23744235.2018.1490813
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发表时间：
2019-01-02
影响因子：
5.8
通讯作者：
Saruki, Nobuhiro
中科院分区：
文献类型：
--
作者：
Hayashi, Toshimasa;Tsukagoshi, Hiroyuki;Saruki, Nobuhiro
猪链球菌（S. suis）是一种重要的新兴人畜共患病病原体。在这里，我们报告了日本群马县养猪户感染猪链球菌的两例。我们使用下一代测序（NGS）根据每个分离株的全基因组测序数据进行了高分辨率分子流行病学分析。 NGS分析显示，两株猪链球菌临床分离株属于血清型2 ST28。系统发育分析表明，至少在1995年之前，两个分离株与从日本猪中分离出的猪链球菌菌株密切相关。由于在两个菌株之间发现了41个核苷酸替换，这些菌株可能源自相同的遗传谱系，但具有不同的散发病例。 NGS 分析是分析细菌感染的强大诊断工具。数据库更加充实，在不久的将来将可以进行更详细的分析。应注意猪链球菌感染，尤其是在畜牧场工作的患者。
Streptococcus suis (S. suis) is an important emerging zoonotic agent. Here, we report two cases of S. suis infection in pig farmers in Gunma Prefecture, Japan. We conducted a high-resolution molecular epidemiologic analysis on the basis of whole-genome sequencing data of each isolate using next-generation sequencing (NGS). NGS analysis revealed that the two S. suis clinical isolates were belonged to serotype 2 ST28. Phylogenetic analysis showed that two isolates were closely related to S. suis strains isolated from pigs in Japan at least until 1995. Since 41 nucleotide substitutions were found between the two strains, these strains might be derived from the same genetic lineage but distinct sporadic cases. NGS analysis is a powerful diagnostic tool for analysing bacterial infections. The database is more fulfilling, and more detailed analysis will become possible in the near future. Attention should be paid to S. suis infections, especially if the patient works on a livestock farm.