Cellular biosensors for drug discovery
Cellular biosensors for drug discovery
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DOI：
10.1016/s0956-5663(01)00173-7
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发表时间：
2001-09-01
影响因子：
12.6
通讯作者：
Negulescu, P
中科院分区：
文献类型：
--
作者：
Durick, K;Negulescu, P
细胞生物学、荧光探针化学、微型化和自动化的最新进展已经允许在各种医疗和工业应用中使用哺乳动物细胞。在这里，我们描述了基于细胞的生物传感器，工程光学报告特定的生物活性的一代。细胞生物传感器由活细胞组成，可用于各种应用，包括筛选药物发现和环境传感的化学库。生物传感器的面板也可以用于阐明新基因的功能。在这里，我们描述了两个例子的建设和使用工程细胞系作为生物传感器的药物发现。(C)2001 Elsevier Science B.V.保留所有权利。
Recent advances in cell biology, fluorescent probe chemistry, miniaturization and automation have allowed the use of mammalian cells in a variety of medical and industrial applications. Here we describe the generation of cell-based biosensors, engineered to optically report specific biological activity. Cellular biosensors are comprised of living cells and can be used in various applications, including screening chemical libraries for drug discovery and environmental sensing. Panels of biosensors may also be useful for elucidating the function of novel genes. Here we describe two examples of the construction and use of engineered cell lines as biosensors for drug discovery. (C) 2001 Elsevier Science B.V. All rights reserved.