Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
Detection of oyster herpesvirus DNA and proteins in asymptomatic Crassostrea gigas adults
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DOI：
10.1016/s0168-1702(02)00007-2
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发表时间：
2002-03-20
期刊：
影响因子：
5
通讯作者：
Gérard, A
中科院分区：
文献类型：
--
作者：
Arzul, I;Renault, T;Gérard, A
自 1972 年以来，世界各地不同双壳类物种中已报道了数起疱疹样病毒感染事件。这些报告大多数涉及死亡率很高的幼虫或幼虫。两例疱疹样病毒病例报告涉及成年牡蛎，即美国的 Crassostrea virginica 和澳大利亚的 Ostrea angasi。最近开发了包括 PCR 和原位杂交 (ISH) 在内的分子技术来检测牡蛎疱疹病毒基因组。在本研究中，使用三种不同的技术对 30 只太平洋牡蛎（Crassostrea gigas）进行了分析：PCR、ISH 和免疫化学，以检测无症状个体中的疱疹病毒。 PCR 和 ISH 分别可以在 93.3% 和 86.6% 的分析牡蛎中检测到牡蛎疱疹病毒 DNA，而多克隆抗体可以在 76.6% 的分析成年牡蛎中检测到病毒蛋白。这些结果表明，牡蛎疱疹病毒以高流行率感染成年牡蛎，并且该病毒在初次感染后可能在其宿主体内持续存在。在几个个体的性腺中检测到病毒 DNA 和病毒蛋白支持了感染可能垂直传播的假设。最后，讨论了本研究中使用的三种技术之间的一致性。 (C) 2002 爱思唯尔科学 B,V。版权所有。
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of analyzed oysters while polyclonal antibodies allowed detection of viral proteins in 76.6% of analyzed adult oysters. These results suggest that oyster herpesvirus infects adult oysters with high prevalence and that the virus may persist in its host after primary infection. The detection of viral DNA and viral proteins in the gonad of several individuals supports the hypothesis of a possible vertical transmission of the infection. Lastly, concordance among the three techniques used in this study is discussed. (C) 2002 Elsevier Science B,V. All rights reserved.