Analysis of molting and metamorphosis in the ecdysteroid-deficient mutant L(3)3DTS of Drosophila melanogaster
Analysis of molting and metamorphosis in the ecdysteroid-deficient mutant L(3)3DTS of Drosophila melanogaster
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黑腹果蝇蜕皮激素缺陷突变体L(3)3DTS的蜕皮和变态分析
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发表时间：
1985
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通讯作者：
J. Gausz
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作者：
J. J. Holden;V. Walker;P. Maróy;K. L. Watson;B. White;J. Gausz
果蝇显性温度敏感突变L(3)3DTS(dts-3)在29℃的限制温度下可致3龄幼虫死亡。温移实验显示出两个明显的温度敏感期，即第三龄幼虫的致死期(可能持续4周)和蛹的后期。在29℃时，突变的成像盘不能在原位生长，但如果在外源蜕皮酮存在的情况下培养或植入野生型幼虫体内，则能正常生长。致死幼虫唯一的形态异常组织是环腺，三龄DTS-3幼虫的前胸腺显著肥大。注射单个野生型环腺挽救了这些突变幼虫，表明突变腺体在功能和形态上都是异常的。最后，突变幼虫的蜕皮激素水平不到野生型的10%。这些结果都与DTS-3幼虫蜕皮类固醇激素产生的损伤是一致的。与其他“无蜕皮激素”突变体的表型进行了比较。
The dominant temperature-sensitive mutation L(3)3DTS (DTS-3) in Drosophila melanogaster causes lethality of heterozygotes during the third larval instar at the restrictive temperature (29°C). Temperature-shift experiments revealed two distinct temperature-sensitive periods, with lethal phases during the third larval instar (which may persist for 4 weeks) and during the late pupal stage. At 29°C mutant imaginal discs are unable to evert in situ, but did evert normally if cultured in the presence of exogenous ecdysterone or when implanted into wild-type larval hosts. The only morphologically abnormal tissue present in the lethal larvae is the ring gland, the prothoracic gland being greatly hypertrophied in third instar DTS-3 larvae. Injection of a single wild-type ring gland rescued these mutant larvae, indicating that the mutant gland is functionally, as well as morphologically, abnormal. Finally, the mutant larvae were shown to have less than 10% of the wild-type ecdysteroid levels. These results are all consistent with a proposed lesion in ecdysteroid hormone production in DTS-3 larvae. A comparison with the phenotypes of other “ecdysone-less” mutants is presented.