Shifts in clostridia, bacteroides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents
Shifts in clostridia, bacteroides and immunoglobulin-coating fecal bacteria associated with weight loss in obese adolescents
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DOI：
10.1038/ijo.2008.260
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发表时间：
2009-07-01
影响因子：
4.9
通讯作者：
Sanz, Y.
中科院分区：
文献类型：
--
作者：
Nadal, I.;Santacruz, A.;Sanz, Y.
目的：评估多学科肥胖治疗方案对超重和肥胖青少年粪便微生物群组成和免疫球蛋白包被细菌的影响及其与减肥的关系。设计：基于热量限制饮食（热量减少= 10-40%）和增加体力活动（热量消耗=每周15-23 kcal/kg体重）的纵向干预研究，为期10周。参与者： 39 名超重和肥胖青少年（BMI 平均值 33.1，范围 23.7-50.4；年龄平均值 14.8，范围 13.0-16.0）。测量：在干预前后测量 BMI、BMI z 分数和血浆生化参数。通过荧光原位杂交分析粪便微生物群。使用荧光标记的 F(ab') 2 抗人 IgA、IgG 和 IgM 检测免疫球蛋白包被细菌。结果：溶组织梭菌和直肠埃希氏菌减少。球状体的比例与整个青少年群体的体重和 BMI z 得分降低显着相关。溶组织梭菌、lituseburense 和直肠埃希氏菌-C. 的比例。对体重减轻超过4公斤的青少年进行干预后，球状菌明显下降，而拟杆菌-普雷沃氏菌组则上升。在同一组青少年中，粪便总能量几乎显着减少，但在减重低于 2.5 公斤的组中则没有。在干预后减重超过 6 公斤的参与者中，IgA 包被的细菌比例也显着下降，同时溶组织梭状芽胞杆菌和直肠埃希氏菌也减少。球状种群。 E.直肠-C。球状菌的比例还与体重减轻超过 4 公斤的参与者的体重减轻和 BMI z 得分降低相关。结论：特定的肠道细菌和相关的 IgA 反应与生活方式干预下青少年的体重变化有关。这些结果表明饮食、肠道微生物群和宿主代谢和肥胖症免疫之间的相互作用。国际肥胖杂志 (2009) 33, 758-767; doi:10.1038/ijo.2008.260； 2008 年 12 月 9 日在线发布
Objective: To evaluate the effects of a multidisciplinary obesity treatment programme on fecal microbiota composition and immunoglobulin-coating bacteria in overweight and obese adolescents and their relationship to weight loss.Design: Longitudinal intervention study based on both a calorie-restricted diet (calorie reduction = 10-40%) and increased physical activity (calorie expenditure = 15-23 kcal/kg body weight per week) for 10 weeks.Participants: Thirty-nine overweight and obese adolescents (BMI mean 33.1 range 23.7-50.4; age mean 14.8 range, 13.0-16.0).Measurements: BMI, BMI z-scores and plasma biochemical parameters were measured before and after the intervention. Fecal microbiota was analyzed by fluorescent in situ hybridization. Immunoglobulin-coating bacteria were detected using fluorescent-labelled F(ab') 2 antihuman IgA, IgG and IgM.Results: Reductions in Clostridium histolyticum and E. rectale-C. coccoides proportions significantly correlated with weight and BMI z-score reductions in the whole adolescent population. Proportions of C. histolyticum, C. lituseburense and E. rectale-C. coccoides dropped significantly whereas those of the Bacteroides-Prevotella group increased after the intervention in those adolescents who lost more than 4 kg. Total fecal energy was almost significantly reduced in the same group of adolescents but not in the group that lost less than 2.5 kg. IgA-coating bacterial proportions also decreased significantly in participants who lost more than 6 kg after the intervention, paralleled to reductions in C. histolyticum and E. rectale-C. coccoides populations. E. rectale-C. coccoides proportions also correlated with weight loss and BMI z-score reduction in participants whose weight loss exceeded 4 kg.Conclusions: Specific gut bacteria and an associated IgA response were related to body weight changes in adolescents under lifestyle intervention. These results suggest interactions between diet, gut microbiota and host metabolism and immunity in obesity. International Journal of Obesity (2009) 33, 758-767; doi:10.1038/ijo.2008.260; published online 9 December 2008