CONFIRMATORY ASSAY FOR THE SIMULTANEOUS DETECTION OF 5 PENICILLINS IN MUSCLE, KIDNEY AND MILK USING LIQUID-CHROMATOGRAPHY - ELECTROSPRAY MASS-SPECTROMETRY
CONFIRMATORY ASSAY FOR THE SIMULTANEOUS DETECTION OF 5 PENICILLINS IN MUSCLE, KIDNEY AND MILK USING LIQUID-CHROMATOGRAPHY - ELECTROSPRAY MASS-SPECTROMETRY
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DOI：
10.1039/an9941902595
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发表时间：
1994-12-01
期刊：
影响因子：
4.2
通讯作者：
KENNEDY, DG
中科院分区：
文献类型：
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作者：
BLANCHFLOWER, WJ;HEWITT, SA;KENNEDY, DG
采用液相色谱-电喷雾质谱法（LC-EMS）同时测定肌肉、肾脏和乳汁中青霉素V和青霉素G沿着苯唑西林、氯唑西林和双氯西林的确证性试验。萘夫西林用作内标物。使用乙腈提取样品，并使用二氯甲烷液液萃取进行净化。将浸提液注入专用LC-EMS仪器中，并使用多个单离子监测检测存在的任何青霉素。每种青霉素可检测到三种或四种离子，符合使用低分辨率质谱法进行确证性残留分析的推荐EU标准之一。检测限低于欧盟规定的每种青霉素的最大残留限度
A confirmatory assay using liquid chromatography-electrospray mass spectrometry (LC-EMS) is presented for the simultaneous determination of penicillins V and G along with oxacillin, cloxacillin and dicloxacillin in muscle, kidney and milk. Nafcillin is used as an internal standard. Samples are extracted using acetonitrile and cleaned up using liquid-liquid extraction into dichloromethane. The extracts are injected into a dedicated LC-EMS instrument and any penicillins present are detected using multiple single-ion monitoring. Three or four ions can be detected for each penicillin, meeting one of the recommended EU criteria for confirmatory residue analyses using low-resolution mass spectrometry. The detection limits are below the stipulated EU maximum residue limits for each of the penicillins