Presence and incidence of food-borne pathogens in Australian chicken litter
Presence and incidence of food-borne pathogens in Australian chicken litter
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DOI：
10.1080/00071668.2010.499424
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Blackall, P. J.
中科院分区：
文献类型：
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作者：
Chinivasagam, H. N.;Redding, M.;Blackall, P. J.
1.在生产周期结束时，从分布在澳大利亚东海岸三个州的28个农场中的每一个农场的单个棚内的散布垫料中收集垫料样品。2. 20份阳性样本的沙门氏菌几何平均值为44 MPN/g。五个样品在100和1000 MPN/g之间，一个在105 MPN/g，表明一系列因素导致了这种生物在窝中的这些不同负载。3. 10份阳性样本的弯曲杆菌几何平均值为30 MPN/g，其中7份样本为100 MPN/g。弯曲杆菌的低流行率和发病率可能是由于这种微生物的快速死亡。4. E.大肠杆菌值明显高于两种主要病原体（几何平均值20 × 105菌落形成单位（cfu）/g），试验中所有样品的总体值或多或少在相同范围内，表明这些微生物在垫料中的贡献模式一致。5.所有样本中均不存在单核细胞增生李斯特菌，该微生物似乎不是垃圾中的问题。6.沙门氏菌血清型中占优势的（70%）是沙门氏菌。索菲亚（从澳大利亚鸡分离的常见血清型），并在所有地区分离。其它主要血清型为S. Virchow和S. Chester（10%）和S. Bovismorbificans和S.婴儿（8%），这些血清型在检测的主要地区显示出空间分布。7.取决于最终用途和相关应用做法和准则的支持，有可能在环境中重新使用废弃物。
1. Litter samples were collected at the end of the production cycle from spread litter in a single shed from each of 28 farms distributed across the three Eastern seaboard States of Australia. 2. The geometric mean for Salmonella was 44 Most Probable Number (MPN)/g for the 20 positive samples. Five samples were between 100 and 1000 MPN/g and one at 105 MPN/g, indicating a range of factors are contributing to these varying loads of this organism in litter. 3. The geometric mean for Campylobacter was 30 MPN/g for the 10 positive samples, with 7 of these samples being 100 MPN/g. The low prevalence and incidence of Campylobacter were possibly due to the rapid die-off of this organism. 4. E. coli values were markedly higher than the two key pathogens (geometric mean 20 x 105 colony forming units (cfu)/g) with overall values being more or less within the same range across all samples in the trial, suggesting a uniform contribution pattern of these organisms in litter. 5. Listeria monocytogenes was absent in all samples and this organism appears not to be an issue in litter. 6. The dominant (70% of the isolates) Salmonella serovar was S. Sofia (a common serovar isolated from chickens in Australia) and was isolated across all regions. Other major serovars were S. Virchow and S. Chester (at 10%) and S. Bovismorbificans and S. Infantis (at 8%) with these serovars demonstrating a spatial distribution across the major regions tested. 7. There is potential to re-use litter in the environment depending on end use and the support of relevant application practices and guidelines.