A new type of tetraploid fish derived via female autotetraploid x male allotetraploid hybridization
A new type of tetraploid fish derived via female autotetraploid x male allotetraploid hybridization
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雌性同源四倍体与雄性异源四倍体杂交衍生的新型四倍体鱼
DOI：
10.1016/j.aquaculture.2020.735244
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发表时间：
2020-07-15
期刊：
影响因子：
4.5
通讯作者：
Liu, Shaojun
中科院分区：
文献类型：
--
作者：
Hu, Fangzhou;Zhong, Haitao;Liu, Shaojun
基于雌性鲫鱼与雌性鲫鱼杂交产生的异源四倍体鱼系（4 nAL，4 n = 200，AABB，F-3-F-28）的生产。红色（RCC，2n = 100，AA）和雄性鲤鱼（Cyprinus carpio（COC，2n = 100，BB）和同源四倍体鱼线（4 nAU，4 n = 200，AAAA，F2-F14）来自雌性RCC与雄性团头鲂（Megalobrama amblycephala）的杂交（BSB，2n = 48），将4 nAU的雌性与4 nAL的雄性杂交，获得了一个新的四倍体杂种（4 nNT，4 n = 200，AAAB）。对4 nNT鱼及其亲本的生物学特性进行了比较研究。DNA含量和染色体数目的测定结果表明，4 nNT为四倍体，具有3套RCC来源的染色体和1套COC来源的染色体。组织切片显示，雌性和雄性4 nNT个体分别具有正常的卵巢和睾丸发育。5SrDNA分子组构分析表明，4 nNT的5SrDNA遗传自原始亲本，并含有明显的碱基变异和序列插入或缺失。新获得的4 nNT杂交鱼具有建立新的四倍体鱼类品系的潜力，也为研究多倍体杂交鱼的基因组结构和遗传表达变异提供了理想的模型。
Based on the production of an allotetraploid fish line (4nAL, 4n = 200, AABB, F-3-F-28) derived from a cross between female Carassius auratus var. red (RCC, 2n = 100, AA) and male Cyprinus carpio (COC, 2n = 100, BB) and an autotetraploid fish line (4nAU, 4n = 200, AAAA, F2-F14) derived from a cross between female RCC and male Megalobrama amblycephala (BSB, 2n = 48), we obtained a new tetraploid hybrid (4nNT, 4n = 200, AAAB) by crossing 4nAU females with 4nAL males. The aim of this study was to comparatively investigate the biological characteristics of 4nNT fish and their parents. The results regarding the DNA content and chromosome numbers indicated that 4nNT were tetraploid with three sets of RCC-derived chromosomes and one set of COC-derived chromosomes. Histological sectioning showed that female and male 4nNT individuals had normal ovarian and testis development, respectively. Analyses of 5S rDNA molecular organization revealed that the 5S rDNA of 4nNT was inherited from the original parents and contained obvious base variations and sequence insertions or deletions. The newly produced 4nNT hybrid has the potential to establish a new tetraploid fish line, and it also provides an ideal model for studying the genomic structure and genetic expression variation in polyploid hybrid fish.