Relationship between functional deficit and severity of experimental fast-strain injury of rat skeletal muscle
Relationship between functional deficit and severity of experimental fast-strain injury of rat skeletal muscle
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DOI：
10.1007/s004210100431
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发表时间：
2001-07-01
影响因子：
3
通讯作者：
Fukuda, H
中科院分区：
文献类型：
--
作者：
Uchiyama, Y;Tamaki, T;Fukuda, H
我们开发了快速拉伤肌肉损伤的大鼠模型，以检查功能缺陷与肌肉损伤程度之间的关系。对成年雄性大鼠的右侧跖肌 (Plt) 施加单一快速应变。通过刺激坐骨神经诱发强直性收缩。通过操纵应变和收缩条件的时间产生三种类型的应变损伤，包括在以下位置施加应变：（1）缩短阶段（SP），（2）强直收缩期间的完全收缩阶段（FCP）和（3）当肌肉不收缩时，即非收缩（NC）。对侧 Plt 肌肉用作对照。进行形态学和功能分析，并通过在施加菌株48小时后测量[H-3]胸苷标记来估计菌株损伤的严重程度。结果显示，施加应变 5 分钟后，所有三组的张力输出均显着下降（P < 0.0 1）。 NC组的功能缺陷持续3周，而SP和FCP组的大鼠在劳损后1周即恢复。 SP和FCP组中[H-3]胸苷摄取的增加相似，但NC中比SP和FCP组高3.5倍(P < 0.0 1)。组织学分析显示，与 SP 和 FCP 组相比，NC 组肌肉间隙增大（P < 0.05）。我们的结果表明，肌纤维的收缩限制了结缔组织损伤的严重程度，并且伴随肌肉结缔组织网络破坏的肌肉损伤需要相对较长的恢复时间。
We developed a rat model of fast-strain muscle injury to examine the relationship between functional deficit and the degree of muscle damage. A single fast strain was applied to the right plantaris (Plt) muscle of adult male rats. A tetanic contraction was induced by stimulating the sciatic nerve. Three types of strain injuries were produced by manipulating the timing of strain and contraction conditions, including applying the strain at: (1) the shortening phase (SP), (2) the full contraction phase (FCP) during tetanic contraction and (3) when the muscle is not contracting, i.e. non-contraction (NC). The contralateral Plt muscle was used as the control. Morphological and functional analysis were performed and the severity of strain injury was estimated by measuring [H-3]thymidine labelling 48 h after the strain was applied. The results show a significant decrease in tension output in all three groups at 5 min after strain application (P < 0.0 1). The functional deficit lasted for 3 weeks in the NC group, while rats of the SP and FCP groups showed recovery 1 week after strain. Increased uptake of [H-3]thymidine was similar in SP and FCP groups, but 3.5-fold higher in NC than in SP and FCP groups (P < 0.0 1). Histological analysis revealed an increase in the size of interstitial spaces of the muscle in NC compared to SP and FCP groups (P < 0.05). Our results suggest that the contraction of muscle fibres limits the severity of connective tissue damage, and that muscle damage accompanied by disruption of the muscle connective tissue network requires a relatively longer recovery time.